Tpk1 Antibody (F-3): sc-374592
- Tpk1 Antibody (F-3) is a mouse monoclonal IgG1 κ Tpk1 antibody, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 367-397 near the C-terminus of Tpk1 of Saccharomyces cerevisiae origin
- Tpk1 Antibody (F-3) is recommended for detection of Tpk1 of y origin by WB, IP, IF and ELISA
- Anti-Tpk1 Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Tpk1 Antibody (F-3) for WB applications. These reagents are now offered in bundles with Tpk1 Antibody (F-3) (see ordering information below).
QUICK LINKS
SEE ALSO...
Tpk1 Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tpk1 of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Tpk1 antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tpk1, also known as Pka1 or Sra3, is a crucial component of the cAMP-dependent protein kinase (PKA) pathway, which plays a vital role in regulating cellular processes such as metabolism, growth, and differentiation in yeast. Tpk1 serves as one of the catalytic subunits of PKA, which is activated by the second messenger cyclic adenosine monophosphate (cAMP). Regulation of cAMP levels is essential for proper signal transduction, as cAMP influences various downstream effects, including gene expression and enzyme activity. Dysregulation of the PKA pathway can lead to significant cellular dysfunction, making Tpk1 a key protein for understanding yeast biology and its implications in broader biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Tpk1 Antibody (F-3) References:
- DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase. | Kataoka, T., et al. 1985. Cell. 43: 493-505. PMID: 2934138
- Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. | Toda, T., et al. 1987. Cell. 50: 277-87. PMID: 3036373
- Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. | Toda, T., et al. 1987. Mol Cell Biol. 7: 1371-7. PMID: 3037314
- The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway. | Broek, D., et al. 1987. Cell. 48: 789-99. PMID: 3545497
- Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins. | Broek, D., et al. 1985. Cell. 41: 763-9. PMID: 3891097
- Interactions between adenylyl cyclase, CAP and RAS from Saccharomyces cerevisiae. | Mintzer, KA. and Field, J. 1994. Cell Signal. 6: 681-94. PMID: 7531994
- Inactivation of the CDC25 gene product in Saccharomyces cerevisiae leads to a decrease in glycolytic activity which is independent of cAMP levels. | Oehlen, LJ., et al. 1993. J Gen Microbiol. 139: 2091-100. PMID: 8245836
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tpk1 Antibody (F-3) | sc-374592 | 200 µg/ml | $322.00 | |||
Tpk1 Antibody (F-3): m-IgG Fc BP-HRP Bundle | sc-527484 | 200 µg Ab; 10 µg BP | $361.00 | |||
Tpk1 Antibody (F-3): m-IgG1 BP-HRP Bundle | sc-532857 | 200 µg Ab; 20 µg BP | $361.00 | |||
Tpk1 Antibody (F-3) AC | sc-374592 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Tpk1 Antibody (F-3) HRP | sc-374592 HRP | 200 µg/ml | $322.00 | |||
Tpk1 Antibody (F-3) FITC | sc-374592 FITC | 200 µg/ml | $336.00 | |||
Tpk1 Antibody (F-3) PE | sc-374592 PE | 200 µg/ml | $349.00 | |||
Tpk1 Antibody (F-3) Alexa Fluor® 488 | sc-374592 AF488 | 200 µg/ml | $364.00 | |||
Tpk1 Antibody (F-3) Alexa Fluor® 546 | sc-374592 AF546 | 200 µg/ml | $364.00 | |||
Tpk1 Antibody (F-3) Alexa Fluor® 594 | sc-374592 AF594 | 200 µg/ml | $364.00 | |||
Tpk1 Antibody (F-3) Alexa Fluor® 647 | sc-374592 AF647 | 200 µg/ml | $364.00 | |||
Tpk1 Antibody (F-3) Alexa Fluor® 680 | sc-374592 AF680 | 200 µg/ml | $364.00 | |||
Tpk1 Antibody (F-3) Alexa Fluor® 790 | sc-374592 AF790 | 200 µg/ml | $364.00 | |||
Tpk1 (F-3) Neutralizing Peptide | sc-374592 P | 100 µg/0.5 ml | $69.00 |