TPD52 CRISPR/Cas9 KO Plasmid (m): sc-423477
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TPD52 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TPD52 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TPD52 CRISPR/Cas9 KO Plasmid (m) | sc-423477 | 20 µg | $397.00 |
Overview
Tpd52 encodes tumor protein D52 (TPD52), a conserved regulator of membrane trafficking and secretory pathway dynamics that influences vesicle transport, exocytosis, and cell surface protein distribution. In mouse cells, TPD52 activity has been linked to processes that support cellular growth and remodeling, including coordination of intracellular membrane compartments and signaling outputs associated with epithelial and immune cell function. Dysregulated TPD52 expression has been reported across multiple cancer-related contexts, where altered trafficking and secretion can affect proliferation, migration, and metabolic adaptation. As a result, Tpd52 is commonly studied in models of tumor biology and cell-state plasticity to define how membrane transport interfaces with oncogenic signaling programs.
TPD52 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tpd52 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tpd52 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tpd52 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TPD52 protein expression.
This CRISPR knockout system enables efficient generation of Tpd52-deficient cell models for investigation of TPD52 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tpd52 exon(s) critical for TPD52 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tpd52 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TPD52 CRISPR/Cas9 KO Plasmid (m) and TPD52 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tpd52 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TPD52 HDR Plasmid (m) and TPD52 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tpd52 homology arms to support homology-directed repair at defined Tpd52 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.