TORC3 Double Nickase Plasmid (h): sc-413035-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TORC3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TORC3 Double Nickase Plasmid (h) and TORC3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CRTC3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TORC3 Antibody (A-12): sc-390712
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TORC3 Double Nickase Plasmid (h) | sc-413035-NIC | 20 µg | $410.00 | |||
TORC3 Double Nickase Plasmid (h2) | sc-413035-NIC-2 | 20 µg | $410.00 |
Human CRTC3 (TORC3) is a CREB-regulated transcription coactivator that integrates cAMP/PKA and calcium-dependent signaling to modulate CREB-driven gene expression programs. Its activity is regulated by phosphorylation-dependent cytoplasmic sequestration and signal-induced nuclear translocation, linking upstream kinase pathways to transcriptional outputs involved in energy balance, mitochondrial metabolism, and stress-responsive gene regulation. CRTC3 contributes to adipocyte and melanocyte biology and has been implicated in altered metabolic phenotypes and melanoma-associated transcriptional states, making it relevant for dissecting context-specific CREB pathway regulation. Studying TORC3 function helps clarify how second-messenger signaling reshapes transcriptional networks across metabolic and oncogenic settings.
TORC3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CRTC3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CRTC3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CRTC3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CRTC3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.