Tomosyn Antibody (15): sc-136105

Tomosyn Antibody (15) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tomosyn protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Tomosyn antibody (15) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). Tomosyn, also known as STXBP5 (syntaxin binding protein 5), LLGL3, or LGL3, is a 1,151 amino acid protein that plays a crucial role in regulating neurotransmitter release and calcium-dependent exocytosis. Tomosyn is primarily located in the cytoplasm, cell junctions, secretory vesicles, and peripheral membrane, where Tomosyn interacts with Syntaxin 1 and Syntaxin 1B. The presence of 14 WD repeats in Tomosyn′s structure is significant, as these motifs facilitate various cellular functions, including chromatin assembly, cell cycle control, and vesicular trafficking. The WD repeats contribute to the formation of a tertiary propeller structure, which is essential for Tomosyn′s regulatory capabilities. Additionally, Tomosyn inhibits membrane fusion and is involved in the assembly of SNARE complexes, which are critical for the fusion of transport vesicles with the plasma membrane. The existence of multiple isoforms of Tomosyn, resulting from alternative splicing events, further highlights the complexity and versatility of Tomosyn′s function in cellular processes.

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Tomosyn Antibody (15) References:

1. Three splicing variants of tomosyn and identification of their syntaxin-binding region.  |  Yokoyama, S., et al. 1999. Biochem Biophys Res Commun. 256: 218-22. PMID: 10066450
2. The WD repeat: a common architecture for diverse functions.  |  Smith, TF., et al. 1999. Trends Biochem Sci. 24: 181-5. PMID: 10322433
3. Tomosyn interacts with the t-SNAREs syntaxin4 and SNAP23 and plays a role in insulin-stimulated GLUT4 translocation.  |  Widberg, CH., et al. 2003. J Biol Chem. 278: 35093-101. PMID: 12832401
4. Identification and characterization of human LLGL4 gene and mouse Llgl4 gene in silico.  |  Katoh, M. and Katoh, M. 2004. Int J Oncol. 24: 737-42. PMID: 14767561
5. Tomosyn inhibits priming of large dense-core vesicles in a calcium-dependent manner.  |  Yizhar, O., et al. 2004. Proc Natl Acad Sci U S A. 101: 2578-83. PMID: 14983051
6. The WD-40 repeat.  |  van der Voorn, L. and Ploegh, HL. 1992. FEBS Lett. 307: 131-4. PMID: 1644165
7. Structural and functional analysis of tomosyn identifies domains important in exocytotic regulation.  |  Williams, AL., et al. 2011. J Biol Chem. 286: 14542-53. PMID: 21330375
8. Tomosyn is a novel Akt substrate mediating insulin-dependent GLUT4 exocytosis.  |  Nagano, K., et al. 2015. Int J Biochem Cell Biol. 62: 62-71. PMID: 25725259
9. Tomosyn guides SNARE complex formation in coordination with Munc18 and Munc13.  |  Li, Y., et al. 2018. FEBS Lett. 592: 1161-1172. PMID: 29485200
10. Tomosyn functions as a PKCδ-regulated fusion clamp in mast cell degranulation.  |  Madera-Salcedo, IK., et al. 2018. Sci Signal. 11: PMID: 29970602
11. Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons.  |  Subkhangulova, A., et al. 2023. Elife. 12: PMID: 37695731
12. Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process.  |  Fujita, Y., et al. 1998. Neuron. 20: 905-15. PMID: 9620695

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tomosyn Antibody (15) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Tomosyn Antibody (15) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tomosyn Antibody (15) Specifications

Alternate Product Name: Tomosyn

Product Clone Name: 15

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 910-1105

Epitope Target: Tomosyn

Epitope Species: rat

Product Reactivity/Detection: Tomosyn

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Tomosyn Antibody (15): AC: Agarose, HRP: Horseradish Peroxidase