Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Tom70 Double Nickase Plasmid (m): sc-424331-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tom70 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Tom70 Double Nickase Plasmid (m) and Tom70 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tomm70a. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tom70 Antibody (A-8): sc-390545
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tom70 Double Nickase Plasmid (m) | sc-424331-NIC | 20 µg | $410.00 |
Mouse Tomm70a encodes Tom70, a mitochondrial outer membrane import receptor that recognizes internal targeting signals and cooperates with chaperones to deliver hydrophobic precursor proteins to the TOM complex for translocation. Tom70 supports mitochondrial proteostasis and bioenergetic capacity by facilitating the import of carrier proteins and other nucleus-encoded mitochondrial components, linking it to oxidative phosphorylation and metabolic adaptation. Perturbation of Tom70-dependent import can promote mitochondrial stress signaling, altered reactive oxygen species handling, and remodeling of apoptosis and innate immune pathways at mitochondria. Dysregulated mitochondrial protein import and outer membrane receptor function are relevant to mechanisms studied in neurodegeneration, cardiometabolic dysfunction, and cancer cell metabolism.
Tom70 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tomm70a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tomm70a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tomm70a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tomm70a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.