Tom20 Antibody (F-10): sc-17764

Tom20 Antibody (F-10) is a mouse monoclonal IgG2a antibody that detects Tom20 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Tom20 antibody (F-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tom20 plays a crucial role in mitochondrial function as a component of the translocase of the outer mitochondrial membrane (TOM) complex, which is essential for the import of nucleus-encoded precursor proteins into mitochondria. Specifically, Tom20 acts as an import receptor that recognizes and binds to these precursor proteins in the cytosol, facilitating their translocation across the mitochondrial outer membrane. This process is vital for maintaining mitochondrial function and energy production, as many proteins required for mitochondrial metabolism and respiration are synthesized in the cytoplasm and must be imported into the mitochondria. The interaction between Tom20 and other components of the TOM complex, such as Tom22 and Tom40, ensures the efficient transport of these proteins, highlighting the importance of Tom20 in cellular energy homeostasis. Tom20 (F-10) monoclonal antibody is specific for mammalian Tom20 protein, making anti-Tom20 antibody (F-10) an invaluable tool for researchers studying mitochondrial dynamics and protein import mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Tom20 Antibody (F-10) References:

1. Distribution of binding sequences for the mitochondrial import receptors Tom20, Tom22, and Tom70 in a presequence-carrying preprotein and a non-cleavable preprotein.  |  Brix, J., et al. 1999. J Biol Chem. 274: 16522-30. PMID: 10347216
2. Biogenesis of Tom40, core component of the TOM complex of mitochondria.  |  Rapaport, D. and Neupert, W. 1999. J Cell Biol. 146: 321-31. PMID: 10427088
3. Tom22 is a multifunctional organizer of the mitochondrial preprotein translocase.  |  van Wilpe, S., et al. 1999. Nature. 401: 485-9. PMID: 10519552
4. The TOM core complex: the general protein import pore of the outer membrane of mitochondria.  |  Ahting, U., et al. 1999. J Cell Biol. 147: 959-68. PMID: 10579717
5. The transport machinery for the import of preproteins across the outer mitochondrial membrane.  |  Ryan, MT., et al. 2000. Int J Biochem Cell Biol. 32: 13-21. PMID: 10661891
6. Mitochondrial protein import. Tom40 plays a major role in targeting and translocation of preproteins by forming a specific binding site for the presequence.  |  Rapaport, D., et al. 1997. J Biol Chem. 272: 18725-31. PMID: 9228044
7. Functional analysis of human mitochondrial receptor Tom20 for protein import into mitochondria.  |  Yano, M., et al. 1998. J Biol Chem. 273: 26844-51. PMID: 9756929
8. Preprotein translocase of the outer mitochondrial membrane: molecular dissection and assembly of the general import pore complex.  |  Dekker, PJ., et al. 1998. Mol Cell Biol. 18: 6515-24. PMID: 9774667

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tom20 Antibody (F-10) has 695 citations in a variety of scientific publications.

Primary antibodies like Anti-Tom20 Antibody (F-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tom20 Antibody (F-10) Specifications

Alternate Product Name: Tom20

Product Clone Name: F-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a lambda light chain

Amino Acids: 1-145

Epitope Target: Tom20

Epitope Species: human

Product Reactivity/Detection: Tom20

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: b

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 695 citations in various scientific publications

Available Conjugates/Forms for Tom20 Antibody (F-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Tom20 antibody is Tom20 Antibody (29): sc-136211.