Tom1L-1 Antibody (3F12): sc-130565

Tom1L-1 Antibody (3F12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Tom1L-1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Tom1L-1 (3F12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tom1L-1, also known as SRCASM or OK/KNS-CL.3, is a 476 amino acid protein localized primarily in the Golgi apparatus and is a crucial component of the multivesicular body (MVB) sorting machinery. Tom1L-1′s strategic location within the cell is vital for its role in the sorting and trafficking of ubiquitinated proteins, which are essential for maintaining cellular homeostasis and regulating signaling pathways. Tom1L-1′s GAT domain binds to ubiquitin, indicating involvement in the recognition and sorting of ubiquitinated substrates into MVBs, thereby influencing various cellular processes, including signal transduction and protein degradation. Additionally, Tom1L-1 interacts with key signaling proteins such as Fyn, GRB2, and PI 3-kinase p85α, and Tom1L-1′s phosphorylation state can modulate these interactions, potentially promoting Fyn activation and impacting downstream signaling cascades. By acting as an adapter protein, Tom1L-1 may play a significant role in the negative regulation of Src family kinases, which are implicated in oncogenic signaling, suggesting a tumor suppressor function by inhibiting the development of squamous cell carcinomas in the skin.

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Tom1L-1 Antibody (3F12) References:

1. 'Srcasm: a novel Src activating and signaling molecule.  |  Seykora, JT., et al. 2002. J Biol Chem. 277: 2812-22. PMID: 11711534
2. Interactions of TOM1L1 with the multivesicular body sorting machinery.  |  Puertollano, R. 2005. J Biol Chem. 280: 9258-64. PMID: 15611048
3. The adaptor protein Tom1L1 is a negative regulator of Src mitogenic signaling induced by growth factors.  |  Franco, M., et al. 2006. Mol Cell Biol. 26: 1932-47. PMID: 16479011
4. Srcasm corrects Fyn-induced epidermal hyperplasia by kinase down-regulation.  |  Li, W., et al. 2007. J Biol Chem. 282: 1161-9. PMID: 17046829
5. The Tom1L1-clathrin heavy chain complex regulates membrane partitioning of the tyrosine kinase Src required for mitogenic and transforming activities.  |  Collin, G., et al. 2007. Mol Cell Biol. 27: 7631-40. PMID: 17785434
6. Recruitment of Tom1L1/Srcasm to endosomes and the midbody by Tsg101.  |  Yanagida-Ishizaki, Y., et al. 2008. Cell Struct Funct. 33: 91-100. PMID: 18367816
7. Odin (ANKS1A) is a Src family kinase target in colorectal cancer cells.  |  Emaduddin, M., et al. 2008. Cell Commun Signal. 6: 7. PMID: 18844995
8. Participation of Tom1L1 in EGF-stimulated endocytosis of EGF receptor.  |  Liu, NS., et al. 2009. EMBO J. 28: 3485-99. PMID: 19798056
9. Srcasm inhibits Fyn-induced cutaneous carcinogenesis with modulation of Notch1 and p53.  |  Zhao, L., et al. 2009. Cancer Res. 69: 9439-47. PMID: 19934324
10. The emerging role of VHS domain-containing Tom1, Tom1L1 and Tom1L2 in membrane trafficking.  |  Wang, T., et al. 2010. Traffic. 11: 1119-28. PMID: 20604899
11. TOM1L1 drives membrane delivery of MT1-MMP to promote ERBB2-induced breast cancer cell invasion.  |  Chevalier, C., et al. 2016. Nat Commun. 7: 10765. PMID: 26899482
12. Fyn and TOM1L1 are recruited to clathrin-coated pits and regulate Akt signaling.  |  Cabral-Dias, R., et al. 2022. J Cell Biol. 221: PMID: 35238864

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tom1L-1 Antibody (3F12) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Tom1L-1 Antibody (3F12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tom1L-1 Antibody (3F12) Specifications

Alternate Product Name: Tom1L-1

Product Clone Name: 3F12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Tom1L-1

Epitope Species: mouse

Product Reactivity/Detection: Tom1L-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Tom1L-1 Antibody (3F12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin