Tollip Antibody (Kimmy-2): sc-59720

Tollip Antibody (Kimmy-2) is a mouse monoclonal IgG1 antibody that detects Tollip protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Tollip antibody (Kimmy-2) is available as the non-conjugated form. Tollip, known as Toll-interacting protein, plays a crucial role in modulating innate immunity signaling by acting as a negative regulator of Toll-like receptor (TLR) pathways. Specifically, Tollip interacts with the serine/threonine kinase IRAK, linking it to the IL-1 receptor complex upon receptor activation, which is vital for controlling the inflammatory response. Overexpression of Tollip in HEK293 cells inhibits NFκB activation in response to TLR2 and TLR4 signaling, thereby limiting the production of proinflammatory mediators during inflammation and infection. Additionally, Tollip forms a complex with Tom1, which is essential for regulating endosomal trafficking of ubiquitinated proteins, highlighting its importance in cellular processes. Tollip exhibits ubiquitous expression in mouse tissues, underscoring its fundamental role in immune regulation across various biological contexts.

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Tollip Antibody (Kimmy-2) References:

1. Tollip, a new component of the IL-1RI pathway, links IRAK to the IL-1 receptor.  |  Burns, K., et al. 2000. Nat Cell Biol. 2: 346-51. PMID: 10854325
2. Cooperation of Toll-like receptor 2 and 6 for cellular activation by soluble tuberculosis factor and Borrelia burgdorferi outer surface protein A lipoprotein: role of Toll-interacting protein and IL-1 receptor signaling molecules in Toll-like receptor 2 signaling.  |  Bulut, Y., et al. 2001. J Immunol. 167: 987-94. PMID: 11441107
3. Negative regulation of toll-like receptor-mediated signaling by Tollip.  |  Zhang, G. and Ghosh, S. 2002. J Biol Chem. 277: 7059-65. PMID: 11751856
4. Tollip and Tom1 form a complex and recruit ubiquitin-conjugated proteins onto early endosomes.  |  Katoh, Y., et al. 2004. J Biol Chem. 279: 24435-43. PMID: 15047686
5. Characterization of Tollip protein upon Lipopolysaccharide challenge.  |  Li, T., et al. 2004. Mol Immunol. 41: 85-92. PMID: 15140579
6. CD26 mediates dissociation of Tollip and IRAK-1 from caveolin-1 and induces upregulation of CD86 on antigen-presenting cells.  |  Ohnuma, K., et al. 2005. Mol Cell Biol. 25: 7743-57. PMID: 16107720
7. Recruitment of clathrin onto endosomes by the Tom1-Tollip complex.  |  Katoh, Y., et al. 2006. Biochem Biophys Res Commun. 341: 143-9. PMID: 16412388
8. Tollip regulates proinflammatory responses to interleukin-1 and lipopolysaccharide.  |  Didierlaurent, A., et al. 2006. Mol Cell Biol. 26: 735-42. PMID: 16428431
9. Association of toll-interacting protein gene polymorphisms with atopic dermatitis.  |  Schimming, TT., et al. 2007. BMC Dermatol. 7: 3. PMID: 17362526
10. Tollip Orchestrates Macrophage Polarization to Alleviate Intestinal Mucosal Inflammation.  |  Liu, X., et al. 2022. J Crohns Colitis. 16: 1151-1167. PMID: 35134154
11. Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy.  |  Jin, S., et al. 2022. Nat Commun. 13: 5204. PMID: 36057605
12. Tegument protein UL21 of alpha-herpesvirus inhibits the innate immunity by triggering CGAS degradation through TOLLIP-mediated selective autophagy.  |  Ma, Z., et al. 2023. Autophagy. 19: 1512-1532. PMID: 36343628

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Tollip Antibody (Kimmy-2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Tollip Antibody (Kimmy-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Tollip Antibody (Kimmy-2) Specifications

Alternate Product Name: Tollip

Product Clone Name: Kimmy-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 1-247

Epitope Target: Tollip

Epitope Species: mouse

Product Reactivity/Detection: Tollip

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Tollip Antibody (Kimmy-2): N/A