Tns2 CRISPR/Cas9 KO Plasmid (h): sc-411726
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tns2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Tns2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tns2 CRISPR/Cas9 KO Plasmid (h) | sc-411726 | 20 µg | $397.00 |
Overview
TNS2 encodes tensin-2 (Tns2), a focal adhesion–associated cytoskeletal adaptor that links integrin receptors to the actin network and organizes signaling at cell–matrix contact sites. Through its modular domains, Tns2 coordinates assembly and turnover of adhesion complexes and modulates pathways involved in cell spreading, migration, and mechanotransduction, including phosphoinositide-dependent signaling and receptor tyrosine kinase–coupled events. Altered TNS2 function has been associated with dysregulated adhesion signaling and tissue homeostasis, with particular relevance to kidney glomerular biology and podocyte integrity. These properties make TNS2 a useful node for studying adhesion-driven signaling, cytoskeletal remodeling, and context-dependent changes in cellular invasiveness and differentiation.
Tns2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TNS2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TNS2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TNS2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Tns2 protein expression.
This CRISPR knockout system enables efficient generation of TNS2-deficient cell models for investigation of Tns2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TNS2 exon(s) critical for Tns2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TNS2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Tns2 CRISPR/Cas9 KO Plasmid (h) and Tns2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TNS2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Tns2 HDR Plasmid (h) and Tns2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TNS2 homology arms to support homology-directed repair at defined TNS2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.