Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TNFα-IP 2 CRISPR/Cas9 KO Plasmid (m): sc-423435
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TNFα-IP 2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TNFα-IP 2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TNFα-IP 2 Antibody (F-6): sc-28318
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TNFα-IP 2 CRISPR/Cas9 KO Plasmid (m) | sc-423435 | 20 µg | $397.00 |
Overview
Tnfaip2 encodes TNFα-induced protein 2 (TNFα-IP 2), a cytokine-responsive factor implicated in inflammatory signaling and remodeling of cell behavior during immune activation. Its expression is induced downstream of TNFα/NF-κB and related pathways, and it has been linked to regulation of cytoskeletal dynamics, membrane trafficking, and migration in myeloid and endothelial contexts. In mouse systems, TNFα-IP 2 is frequently used as a readout of pro-inflammatory transcriptional programs and as a node connecting innate immune cues to changes in adhesion and motility. Dysregulated TNFα-IP 2-associated signaling has been studied in the context of chronic inflammation and tumor-associated microenvironment biology, supporting its utility in dissecting inflammation–cancer crosstalk without implying clinical outcomes.
TNFα-IP 2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnfaip2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnfaip2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnfaip2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TNFα-IP 2 protein expression.
This CRISPR knockout system enables efficient generation of Tnfaip2-deficient cell models for investigation of TNFα-IP 2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnfaip2 exon(s) critical for TNFα-IP 2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnfaip2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TNFα-IP 2 CRISPR/Cas9 KO Plasmid (m) and TNFα-IP 2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnfaip2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TNFα-IP 2 HDR Plasmid (m) and TNFα-IP 2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnfaip2 homology arms to support homology-directed repair at defined Tnfaip2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.