TNFα-IP 1 CRISPR/Cas9 KO Plasmid (m): sc-423434
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TNFα-IP 1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TNFα-IP 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TNFα-IP 1 CRISPR/Cas9 KO Plasmid (m) | sc-423434 | 20 µg | $397.00 |
Overview
Tnfaip1 (TNFα-IP 1) encodes a TNFα-inducible protein implicated in stress-responsive signaling and cellular homeostasis. Reported functions include roles in nuclear and cytoplasmic processes that influence cell-cycle progression, DNA damage responses, and protein turnover, linking TNF/NF-κB–associated inflammatory cues to downstream transcriptional and survival programs. In mouse systems, TNFα-IP 1 is studied for its contribution to context-dependent regulation of proliferation and apoptosis, processes that shape tissue remodeling and immune microenvironments. Dysregulation of these pathways is frequently examined in models of inflammatory disease biology and tumor-associated signaling without implying clinical outcomes.
TNFα-IP 1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnfaip1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnfaip1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnfaip1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TNFα-IP 1 protein expression.
This CRISPR knockout system enables efficient generation of Tnfaip1-deficient cell models for investigation of TNFα-IP 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnfaip1 exon(s) critical for TNFα-IP 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnfaip1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TNFα-IP 1 CRISPR/Cas9 KO Plasmid (m) and TNFα-IP 1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnfaip1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TNFα-IP 1 HDR Plasmid (m) and TNFα-IP 1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnfaip1 homology arms to support homology-directed repair at defined Tnfaip1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.