Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TMTC3 Double Nickase Plasmid (h): sc-409056-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMTC3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TMTC3 Double Nickase Plasmid (h) and TMTC3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TMTC3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TMTC3 Antibody (G-9): sc-398137
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMTC3 Double Nickase Plasmid (h) | sc-409056-NIC | 20 µg | $410.00 |
TMTC3 encodes an endoplasmic reticulum–resident transmembrane tetratricopeptide repeat protein implicated in protein quality control and glycoprotein maturation, including regulation of O-mannosylation on select substrates. Through its role in ER proteostasis, TMTC3 influences folding, trafficking, and stability of membrane and secreted proteins, intersecting with pathways such as ER stress signaling and unfolded protein response modulation. Genetic and functional studies have linked TMTC3 perturbation to neurodevelopmental phenotypes and altered cellular homeostasis consistent with disrupted protein processing. As a result, TMTC3 is frequently investigated in models of neuronal development, adhesion and signaling receptor maturation, and ER-associated pathways relevant to congenital and neurological disease mechanisms.
TMTC3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TMTC3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TMTC3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TMTC3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TMTC3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.