TMEM88 CRISPR/Cas9 KO Plasmid (h): sc-405789
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMEM88 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TMEM88 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM88 CRISPR/Cas9 KO Plasmid (h) | sc-405789 | 20 µg | $397.00 |
Overview
TMEM88 encodes a small transmembrane protein implicated in the regulation of Wnt signaling by modulating Dishevelled-dependent signal transduction, influencing β-catenin–driven transcriptional programs. Through its effects on pathway output, TMEM88 can shape cellular processes such as proliferation, differentiation, polarity, and epithelial–mesenchymal dynamics. Altered TMEM88 expression or pathway context has been associated with dysregulated Wnt activity across multiple disease-relevant settings, including cancer and tissue remodeling. In human model systems, TMEM88 perturbation is used to interrogate membrane-proximal control points that tune Wnt pathway amplitude and downstream gene expression networks.
TMEM88 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TMEM88 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TMEM88 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TMEM88 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TMEM88 protein expression.
This CRISPR knockout system enables efficient generation of TMEM88-deficient cell models for investigation of TMEM88 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TMEM88 exon(s) critical for TMEM88 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TMEM88 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TMEM88 CRISPR/Cas9 KO Plasmid (h) and TMEM88 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TMEM88 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TMEM88 HDR Plasmid (h) and TMEM88 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TMEM88 homology arms to support homology-directed repair at defined TMEM88 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.