TMEM63A CRISPR/Cas9 KO Plasmid (h): sc-411328
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMEM63A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TMEM63A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM63A CRISPR/Cas9 KO Plasmid (h) | sc-411328 | 20 µg | $397.00 |
Overview
TMEM63A encodes a multi-pass transmembrane protein proposed to function as an osmosensitive mechanosensitive ion channel, linking changes in membrane tension and cell volume to ionic homeostasis. It has been implicated in mechanotransduction-related processes that influence intracellular signaling, membrane excitability, and stress adaptation, with potential crosstalk to calcium-dependent pathways. Expression patterns and emerging functional studies suggest relevance to sensory physiology and neural or epithelial contexts where osmotic and mechanical cues are prominent. Dysregulation of membrane transport and mechanosensing programs is broadly associated with neurological and homeostatic disorders, making TMEM63A a useful target for interrogating these mechanisms in cellular models.
TMEM63A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TMEM63A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TMEM63A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TMEM63A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TMEM63A protein expression.
This CRISPR knockout system enables efficient generation of TMEM63A-deficient cell models for investigation of TMEM63A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TMEM63A exon(s) critical for TMEM63A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TMEM63A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TMEM63A CRISPR/Cas9 KO Plasmid (h) and TMEM63A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TMEM63A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TMEM63A HDR Plasmid (h) and TMEM63A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TMEM63A homology arms to support homology-directed repair at defined TMEM63A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.