Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TMEM205 CRISPR/Cas9 KO Plasmid (m): sc-433421
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMEM205 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TMEM205 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TMEM205 Antibody (B-5): sc-514568
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM205 CRISPR/Cas9 KO Plasmid (m) | sc-433421 | 20 µg | $397.00 |
Overview
Tmem205 encodes TMEM205, a multi-pass membrane protein implicated in cellular transport processes at the plasma membrane and endomembrane system, with reported roles in regulating intracellular accumulation and efflux of xenobiotics and metal-containing compounds. TMEM205 has been linked to stress-adaptive remodeling of membrane trafficking and vesicular transport, processes that intersect with proteostasis and cellular response to cytotoxic insults. Altered TMEM205 expression has been associated with drug response phenotypes in cancer models, making it a useful target for dissecting mechanisms of chemoresistance and membrane transport regulation. In mouse systems, Tmem205 perturbation can help clarify how transporter-like membrane proteins influence cellular homeostasis and sensitivity to environmental challenges.
TMEM205 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tmem205 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tmem205 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tmem205 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TMEM205 protein expression.
This CRISPR knockout system enables efficient generation of Tmem205-deficient cell models for investigation of TMEM205 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tmem205 exon(s) critical for TMEM205 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tmem205 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TMEM205 CRISPR/Cas9 KO Plasmid (m) and TMEM205 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tmem205 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TMEM205 HDR Plasmid (m) and TMEM205 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tmem205 homology arms to support homology-directed repair at defined Tmem205 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.