TMEM14B CRISPR/Cas9 KO Plasmid (h): sc-418197
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMEM14B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TMEM14B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM14B CRISPR/Cas9 KO Plasmid (h) | sc-418197 | 20 µg | $397.00 |
Overview
TMEM14B encodes a predicted multi-pass transmembrane protein enriched in intracellular membranes and linked to mitochondrial homeostasis and membrane-associated metabolic processes. As a member of the TMEM14 family, it is frequently studied in the context of organelle dynamics, redox balance, and regulation of apoptosis under cellular stress conditions. Altered TMEM14B expression has been reported in transcriptomic signatures across multiple cancer types and other proliferative states, suggesting relevance to pathways that couple mitochondrial function to cell growth and survival. These features make TMEM14B a useful target for dissecting mitochondrial-linked signaling networks and stress adaptation programs in human cells.
TMEM14B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TMEM14B gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TMEM14B together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TMEM14B open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TMEM14B protein expression.
This CRISPR knockout system enables efficient generation of TMEM14B-deficient cell models for investigation of TMEM14B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TMEM14B exon(s) critical for TMEM14B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TMEM14B genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TMEM14B CRISPR/Cas9 KO Plasmid (h) and TMEM14B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TMEM14B locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TMEM14B HDR Plasmid (h) and TMEM14B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TMEM14B homology arms to support homology-directed repair at defined TMEM14B target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.