TMEM12 Antibody (D-2): sc-398948
- TMEM12 Antibody (D-2) is a mouse monoclonal IgG2a κ TMEM12 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 205-223 at the C-terminus of TMEM12 of human origin
- TMEM12 Antibody (D-2) is recommended for detection of TMEM12 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TMEM12 Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TMEM12 Antibody (D-2) for WB applications. These reagents are now offered in bundles with TMEM12 Antibody (D-2) (see ordering information below).
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TMEM12 Antibody (D-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects TMEM12 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TMEM12 (D-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TMEM12 protein, also known as USH3AL1 or CLRN3, is a 226 amino acid multi-pass membrane protein that plays a crucial role in maintaining sensory cells, particularly in the inner ear and retina. TMEM12 protein is involved in forming and functioning excitatory ribbon synapse junctions between hair cells and cochlear ganglion cells, as well as similar synapses in the retina. This function is vital for proper auditory and visual processing, and defects in TMEM12-encoding genes can lead to Usher syndrome type 3, a condition characterized by progressive hearing loss and retinitis pigmentosa. Two alternatively spliced isoforms of TMEM12 suggest diverse functional roles, further emphasizing TMEM12 protein′s importance in sensory cell integrity and communication.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TMEM12 Antibody (D-2) References:
- Possible interaction between USH1B and USH3 gene products as implied by apparent digenic deafness inheritance. | Adato, A., et al. 1999. Am J Hum Genet. 65: 261-5. PMID: 10364543
- A sequence-ready map of the Usher syndrome type III critical region on chromosome 3q. | Joensuu, T., et al. 2000. Genomics. 63: 409-16. PMID: 10704288
- Mutations in a novel gene with transmembrane domains underlie Usher syndrome type 3. | Joensuu, T., et al. 2001. Am J Hum Genet. 69: 673-84. PMID: 11524702
- USH3A transcripts encode clarin-1, a four-transmembrane-domain protein with a possible role in sensory synapses. | Adato, A., et al. 2002. Eur J Hum Genet. 10: 339-50. PMID: 12080385
- Usher syndrome type III: revised genomic structure of the USH3 gene and identification of novel mutations. | Fields, RR., et al. 2002. Am J Hum Genet. 71: 607-17. PMID: 12145752
- Retinal disease in Usher syndrome III caused by mutations in the clarin-1 gene. | Herrera, W., et al. 2008. Invest Ophthalmol Vis Sci. 49: 2651-60. PMID: 18281613
- Assignment of an Usher syndrome type III (USH3) gene to chromosome 3q. | Sankila, EM., et al. 1995. Hum Mol Genet. 4: 93-8. PMID: 7711740
- Refined mapping of the Usher syndrome type III locus on chromosome 3, exclusion of candidate genes, and identification of the putative mouse homologous region. | Joensuu, T., et al. 1996. Genomics. 38: 255-63. PMID: 8975700
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMEM12 Antibody (D-2) | sc-398948 | 200 µg/ml | $322.00 | |||
TMEM12 Antibody (D-2): m-IgG Fc BP-HRP Bundle | sc-530986 | 200 µg Ab; 10 µg BP | $361.00 | |||
TMEM12 Antibody (D-2): m-IgGκ BP-HRP Bundle | sc-524553 | 200 µg Ab, 40 µg BP | $361.00 | |||
TMEM12 Antibody (D-2): m-IgG2a BP-HRP Bundle | sc-547654 | 200 µg Ab; 10 µg BP | $361.00 | |||
TMEM12 Antibody (D-2) AC | sc-398948 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TMEM12 Antibody (D-2) HRP | sc-398948 HRP | 200 µg/ml | $322.00 | |||
TMEM12 Antibody (D-2) FITC | sc-398948 FITC | 200 µg/ml | $336.00 | |||
TMEM12 Antibody (D-2) PE | sc-398948 PE | 200 µg/ml | $349.00 | |||
TMEM12 Antibody (D-2) Alexa Fluor® 488 | sc-398948 AF488 | 200 µg/ml | $364.00 | |||
TMEM12 Antibody (D-2) Alexa Fluor® 546 | sc-398948 AF546 | 200 µg/ml | $364.00 | |||
TMEM12 Antibody (D-2) Alexa Fluor® 594 | sc-398948 AF594 | 200 µg/ml | $364.00 | |||
TMEM12 Antibody (D-2) Alexa Fluor® 647 | sc-398948 AF647 | 200 µg/ml | $364.00 | |||
TMEM12 Antibody (D-2) Alexa Fluor® 680 | sc-398948 AF680 | 200 µg/ml | $364.00 | |||
TMEM12 Antibody (D-2) Alexa Fluor® 790 | sc-398948 AF790 | 200 µg/ml | $364.00 | |||
TMEM12 (D-2) Neutralizing Peptide | sc-398948 P | 100 µg/0.5 ml | $69.00 |