TMED2 Antibody (E-12): sc-376458

TMED2 Antibody (E-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects TMED2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TMED2 (E-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TMED2, also known as transmembrane emp24 domain trafficking protein 2, P24A, or RNP24, is a 201 amino acid protein that belongs to the EMP24/GP25L family and is characterized as a single-pass type I membrane protein containing a GOLD domain. The GOLD domain, which spans approximately 90 to 150 amino acids, is crucial for mediating protein-protein interactions and plays a significant role in the dynamics of the Golgi apparatus. This domain facilitates interactions with lipids, sterols, and fatty acids, as well as with RUN domains that associate with cytoskeletal filaments of membrane proteins. TMED2 inhibits the GTPase-activating activity of ARFGAP1, thereby contributing to the budding of coatomer-coated and other types of coated vesicles. Additionally, as part of a complex with SURF-4 and TMP21, TMED2 is instrumental in binding cargo molecules, which are essential for their incorporation into budding vesicles, highlighting its importance in intracellular trafficking and protein sorting.

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TMED2 Antibody (E-12) References:

1. The iodocyanopindolol and SM-11044 binding protein belongs to the TM9SF multispanning membrane protein superfamily.  |  Sugasawa, T., et al. 2001. Gene. 273: 227-37. PMID: 11595169
2. Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus.  |  Barr, FA., et al. 2001. J Cell Biol. 155: 885-91. PMID: 11739402
3. Cell-type-specific and selectively induced expression of members of the p24 family of putative cargo receptors.  |  Rötter, J., et al. 2002. J Cell Sci. 115: 1049-58. PMID: 11870223
4. The GOLD domain, a novel protein module involved in Golgi function and secretion.  |  Anantharaman, V. and Aravind, L. 2002. Genome Biol. 3: research0023. PMID: 12049664
5. p24A, a type I transmembrane protein, controls ARF1-dependent resensitization of protease-activated receptor-2 by influence on receptor trafficking.  |  Luo, W., et al. 2007. J Biol Chem. 282: 30246-55. PMID: 17693410
6. The cargo receptors Surf4, endoplasmic reticulum-Golgi intermediate compartment (ERGIC)-53, and p25 are required to maintain the architecture of ERGIC and Golgi.  |  Mitrovic, S., et al. 2008. Mol Biol Cell. 19: 1976-90. PMID: 18287528
7. The trafficking protein Tmed2/p24beta(1) is required for morphogenesis of the mouse embryo and placenta.  |  Jerome-Majewska, LA., et al. 2010. Dev Biol. 341: 154-66. PMID: 20178780
8. Increased expression of TMED2 is an unfavorable prognostic factor in patients with breast cancer.  |  Lin, X., et al. 2019. Cancer Manag Res. 11: 2203-2214. PMID: 31114314
9. Expression and Importance of TMED2 in Multiple Myeloma Cells.  |  Ge, X., et al. 2020. Cancer Manag Res. 12: 12895-12903. PMID: 33364837
10. Tmp21 and p24A, two type I proteins enriched in pancreatic microsomal membranes, are members of a protein family involved in vesicular trafficking.  |  Blum, R., et al. 1996. J Biol Chem. 271: 17183-9. PMID: 8663407
11. gp25L/emp24/p24 protein family members of the cis-Golgi network bind both COP I and II coatomer.  |  Dominguez, M., et al. 1998. J Cell Biol. 140: 751-65. PMID: 9472029
12. Intracellular localization and in vivo trafficking of p24A and p23.  |  Blum, R., et al. 1999. J Cell Sci. 112 (Pt 4): 537-48. PMID: 9914165

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TMED2 Antibody (E-12) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-TMED2 Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TMED2 Antibody (E-12) Specifications

Alternate Product Name: TMED2

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 94-201

Epitope Target: TMED2

Epitope Species: human

Product Reactivity/Detection: TMED2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for TMED2 Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin