TMC8 CRISPR/Cas9 KO Plasmid (h): sc-406378
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMC8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TMC8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMC8 CRISPR/Cas9 KO Plasmid (h) | sc-406378 | 20 µg | $397.00 |
Overview
TMC8 (transmembrane channel-like 8; also known as EVER2) encodes a multi-pass membrane protein implicated in regulation of keratinocyte and immune cell signaling, with reported roles in controlling intracellular zinc homeostasis and downstream transcriptional responses. It has been linked to pathways influencing antiviral defense, epidermal differentiation, and inflammatory signaling, including modulation of NF-κB–associated responses in certain contexts. Genetic perturbation of TMC8 is associated with susceptibility to epidermodysplasia verruciformis and altered host control of cutaneous human papillomavirus infections, supporting its relevance in skin barrier biology and virus–host interaction studies. As a membrane-associated factor, TMC8 is frequently investigated for how ion-dependent signaling interfaces with epithelial immunity and persistence of viral infection.
TMC8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TMC8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TMC8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TMC8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TMC8 protein expression.
This CRISPR knockout system enables efficient generation of TMC8-deficient cell models for investigation of TMC8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TMC8 exon(s) critical for TMC8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TMC8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TMC8 CRISPR/Cas9 KO Plasmid (h) and TMC8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TMC8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TMC8 HDR Plasmid (h) and TMC8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TMC8 homology arms to support homology-directed repair at defined TMC8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.