TLX3 Antibody (G-8): sc-514691

TLX3 Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects TLX3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TLX3 (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TLX3 plays a crucial role in the development of the central nervous system, particularly in the formation of first-order relay visceral sensory neurons in the brainstem. TLX3 expression is vital for the proper development of noradrenergic centers, as evidenced by the compromised development observed in TLX3-deficient mice, which exhibit severe respiratory issues and often succumb shortly after birth due to central respiratory failure. Additionally, TLX3 is implicated in T-cell acute lymphoblastic leukemia (T-ALL), where the t(5;14)(q35;q32) translocation leads to increased transcription of TLX3 gene, highlighting TLX3′s significance in both developmental biology and cancer research. TLX3 gene is located on chromosome 5q34-q35, further emphasizing TLX3′s importance in genetic studies and potential therapeutic targets.

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TLX3 Antibody (G-8) References:

1. Rnx deficiency results in congenital central hypoventilation.  |  Shirasawa, S., et al. 2000. Nat Genet. 24: 287-90. PMID: 10700185
2. Assignment of the HOX11L2 gene to human chromosome band 5q35.1 and of its murine homolog to mouse chromosome bands 11A4-A5 by in situ hybridization.  |  Cinti, R., et al. 2001. Cytogenet Cell Genet. 92: 354-5. PMID: 11435716
3. Assignment of the human homeobox 11-like 2 gene (HOX11L2) to chromosome 5q34-->q35 by radiation hybrid mapping.  |  Lee-Kirsch, MA., et al. 2001. Cytogenet Cell Genet. 92: 358. PMID: 11435718
4. Formation of brainstem (nor)adrenergic centers and first-order relay visceral sensory neurons is dependent on homeodomain protein Rnx/Tlx3.  |  Qian, Y., et al. 2001. Genes Dev. 15: 2533-45. PMID: 11581159
5. A new recurrent and specific cryptic translocation, t(5;14)(q35;q32), is associated with expression of the Hox11L2 gene in T acute lymphoblastic leukemia.  |  Bernard, OA., et al. 2001. Leukemia. 15: 1495-504. PMID: 11587205
6. Tlx3 and Tlx1 are post-mitotic selector genes determining glutamatergic over GABAergic cell fates.  |  Cheng, L., et al. 2004. Nat Neurosci. 7: 510-7. PMID: 15064766
7. HOX11L2/TLX3 is transcriptionally activated through T-cell regulatory elements downstream of BCL11B as a result of the t(5;14)(q35;q32).  |  Su, XY., et al. 2006. Blood. 108: 4198-201. PMID: 16926283
8. Tlx1 and Tlx3 coordinate specification of dorsal horn pain-modulatory peptidergic neurons.  |  Xu, Y., et al. 2008. J Neurosci. 28: 4037-46. PMID: 18400903
9. Tlx3 controls cholinergic transmitter and Peptide phenotypes in a subset of prenatal sympathetic neurons.  |  Huang, T., et al. 2013. J Neurosci. 33: 10667-75. PMID: 23804090
10. Dual role of Tlx3 as modulator of Prrxl1 transcription and phosphorylation.  |  Regadas, I., et al. 2014. Biochim Biophys Acta. 1839: 1121-31. PMID: 25138281
11. Homeobox protein TLX3 activates miR-125b expression to promote T-cell acute lymphoblastic leukemia.  |  Renou, L., et al. 2017. Blood Adv. 1: 733-747. PMID: 29296717
12. MRD-risk stratification mitigates TLX3 prognostic impact in paediatric T-cell acute lymphoblastic leukaemia: A national cohort analysis.  |  Trinquand, A., et al. 2024. Br J Haematol. 205: 729-732. PMID: 38937136

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TLX3 Antibody (G-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-TLX3 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TLX3 Antibody (G-8) Specifications

Alternate Product Name: TLX3

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 31-85

Epitope Target: TLX3

Epitope Species: human

Product Reactivity/Detection: TLX3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for TLX3 Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent