TLR4 Antibody (HTA125) recommended for detection of TLR4/MD-2 complex of human origin by IP, IF, IHC(P) and FCM

TLR4 Antibody (HTA125): sc-13593

TLR4 Antibody (HTA125) is rated 4.8 out of 5 by 12.
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Datasheets

    • TLR4 Antibody (HTA125) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against Toll-like receptor 4 (TLR4)
    • TLR4 Antibody (HTA125) is recommended for detection of TLR4/MD-2 complex of human origin by IP, IF, IHC(P) and FCM
    • Anti-TLR4 Antibody (HTA125) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • also provided azide-free for biological studies, sc-13593 L, 200 µg/0.1 ml
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TLR4 (HTA125): sc-13593.

How can you vote for your own antibody? Where are the references for all your comments?

Asked by: PaulPandora
Thank you very much for your question. We currently list 36 product citations from peer-reviewed journals for this antibody. The product reviews and ratings on our website for this antibody are provided either by customers, screened from select product citations or provided by our laboratory staff using this product e.g. in routine QC applications. The reviews are marked accordingly i.e. by customer nick name, individual SCBT QC employees or SCBT Publication Review. We feel, that this type of information might be useful for existing or potential customers researching this product.
Answered by: Technical Support Europe
Date published: 2017-09-04

Is TLR4 (HTA125) suitable for use in Western blotting of mouse samples?

Asked by: cjMara
No. We do not recommend this antibody for use in WB or for mouse samples. We would recommend an alternative mouse monoclonal antibody, sc-293072: TLR4 (25) for this application.
Answered by: Technical Support 15
Date published: 2017-01-22
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Rated 3 out of 5 by from This antibody is a good with immunohistochemistry This antibody works with immunohistochemistry with human tissue.
Date published: 2020-06-12
Rated 5 out of 5 by from Very good antibody for western blots We probed human and pig leukocyte samples. Detected a single band of expected molecular weight.
Date published: 2019-04-12
Rated 4 out of 5 by from Works well in WB application We analyzed human and mice leukocytes samples by WB
Date published: 2019-03-27
Rated 5 out of 5 by from 效果很好 之前买过抗体,跑出来结果很好,这次准备还要再订购几个抗体,不仅质量好,价格也相对优惠,给我的实验提供很多帮助
Date published: 2018-01-04
Rated 5 out of 5 by from Excellent TLR4 antibody! This antibody works great for detecting TLR4 by IHC and even flow cytometry. It is very specific and gives a great signal. It has been published with more than 30 times.
Date published: 2017-01-11
Rated 5 out of 5 by from Great IHC staining IHC staining results are excellent; very pleased !!
Date published: 2017-01-05
Rated 5 out of 5 by from This antibody is very good quality in WB and IHC This antibody is very good quality in WB and IHC.<br /> It can get very good result.I suggest WB:1:200 dilution. IHC:1:50 dilution.
Date published: 2015-11-24
Rated 5 out of 5 by from Works very well for flow cytometry with U Works very well for flow cytometry with U-937 and THP-1 samples. -SCBT QC
Date published: 2015-03-30
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