TLR2 CRISPR Activation Plasmid (h): sc-400267-ACT. Western blot analysis of TLR2 expression in non-transfected control (A) and TLR2 CRISPR Activation Plasmid transfected (B) HEK293T whole cell lysates. Blot was probed with TLR2 (A-9): sc-166900. α-actinin (H-2): sc-17829 used as specificity and loading control.
TLR2 CRISPR/Cas9 KO Plasmid (h): sc-400267
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TLR2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TLR2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- TLR2 HDR Plasmid (h) (sc-400267-HDR) is recommended for co-transfection with TLR2 CRISPR/Cas9 KO Plasmid (h) to enable selection of successfully edited cells through HDR-mediated integration of a puromycin resistance cassette and RFP reporter gene
- TLR2 HDR Plasmid (h) is a pool of plasmids, each containing a homology-directed repair (HDR) template corresponding to the gRNA target sites in the TLR2 CRISPR/Cas9 KO Plasmid (h)
- Each HDR plasmid contains two ~800 bp homology arms flanking the puromycin resistance and RFP cassettes, designed to bind genomic DNA sequences surrounding the Cas9-induced double-strand break site and facilitate precise HDR-mediated integration
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TLR2 Antibody (TL2.1): sc-21759
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TLR2 CRISPR/Cas9 KO Plasmid (h) | sc-400267 | 20 µg | $397.00 | |||
TLR2 HDR Plasmid (h) | sc-400267-HDR | 20 µg | $445.00 |