TIP60 Antibody (C-7): sc-166323

TIP60 Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects TIP60 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-TIP60 antibody (C-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TIP60 plays a crucial role in chromatin remodeling and transcription regulation as a chromatin-associated histone acetyltransferase (HAT). TIP60 function is particularly important in gene expression, as TIP60 facilitates histone acetylation, leading to a more relaxed chromatin structure that allows for transcriptional activation. Dysregulation of TIP60 has been implicated in various cancers, particularly due to TIP60′s involvement in fusion protein formation resulting from chromosomal translocations in acute myeloid leukemia. These fusion proteins enhance transcriptional activation through aberrant histone acetylation, highlighting TIP60′s significance in maintaining normal cellular functions and potential as a therapeutic target. TIP60 interacts with other proteins such as MOZ and MORF, which share functional similarities and contribute to the complex regulation of transcriptional processes.

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TIP60 Antibody (C-7) References:

1. Tip60 is a nuclear hormone receptor coactivator.  |  Brady, ME., et al. 1999. J Biol Chem. 274: 17599-604. PMID: 10364196
2. Identification of a human histone acetyltransferase related to monocytic leukemia zinc finger protein.  |  Champagne, N., et al. 1999. J Biol Chem. 274: 28528-36. PMID: 10497217
3. Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis.  |  Song, X., et al. 2021. Nat Chem Biol. 17: 1314-1323. PMID: 34608293
4. Loss of TIP60 (KAT5) abolishes H2AZ lysine 7 acetylation and causes p53, INK4A, and ARF-independent cell cycle arrest.  |  Wichmann, J., et al. 2022. Cell Death Dis. 13: 627. PMID: 35853868
5. Experience-dependent Tip60 nucleocytoplasmic transport is regulated by its NLS/NES sequences for neuroplasticity gene control.  |  Armour, EM., et al. 2023. Mol Cell Neurosci. 127: 103888. PMID: 37598897
6. 3, 3'-diindolylmethane, a natural aryl hydrocarbon receptor agonist, alleviates ulcerative colitis by enhancing 'glycolysis-lactate-STAT3″ and TIP60 signals-mediated Treg differentiation.  |  Liu, S., et al. 2023. Mol Immunol. 163: 147-162. PMID: 37793204
7. The translocation t(8;16)(p11;p13) of acute myeloid leukaemia fuses a putative acetyltransferase to the CREB-binding protein.  |  Borrow, J., et al. 1996. Nat Genet. 14: 33-41. PMID: 8782817
8. mof, a putative acetyl transferase gene related to the Tip60 and MOZ human genes and to the SAS genes of yeast, is required for dosage compensation in Drosophila.  |  Hilfiker, A., et al. 1997. EMBO J. 16: 2054-60. PMID: 9155031
9. Abnormalities of chromosome band 8p11 in leukemia: two clinical syndromes can be distinguished on the basis of MOZ involvement.  |  Aguiar, RC., et al. 1997. Blood. 90: 3130-5. PMID: 9376594
10. Novel substrate specificity of the histone acetyltransferase activity of HIV-1-Tat interactive protein Tip60.  |  Yamamoto, T. and Horikoshi, M. 1997. J Biol Chem. 272: 30595-8. PMID: 9388189
11. A novel fusion between MOZ and the nuclear receptor coactivator TIF2 in acute myeloid leukemia.  |  Carapeti, M., et al. 1998. Blood. 91: 3127-33. PMID: 9558366
12. Roles of histone acetyltransferases and deacetylases in gene regulation.  |  Kuo, MH. and Allis, CD. 1998. Bioessays. 20: 615-26. PMID: 9780836

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TIP60 Antibody (C-7) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-TIP60 Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TIP60 Antibody (C-7) Specifications

Alternate Product Name: TIP60

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 421-513

Epitope Target: TIP60

Epitope Species: human

Product Reactivity/Detection: TIP60

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for TIP60 Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin