TIP39 CRISPR/Cas9 KO Plasmid (h): sc-405791
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TIP39 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TIP39 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TIP39 CRISPR/Cas9 KO Plasmid (h) | sc-405791 | 20 µg | $397.00 |
Overview
Parathyroid hormone 2 (PTH2) encodes tuberoinfundibular peptide of 39 residues (TIP39), a neuropeptide that functions as an endogenous ligand for parathyroid hormone receptor 2 (PTH2R), a class B GPCR. TIP39–PTH2R signaling couples predominantly to cAMP/PKA and can engage calcium-dependent pathways, shaping neuronal excitability and neuroendocrine outputs in hypothalamic and limbic circuits. In human biology, PTH2/TIP39 has been linked to modulation of nociception, stress responses, thermoregulation, and reproductive axis control through neuropeptidergic communication. Dysregulation of these pathways provides a mechanistic context for studying sensory processing and neuroendocrine phenotypes in disease-relevant models.
TIP39 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PTH2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PTH2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PTH2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TIP39 protein expression.
This CRISPR knockout system enables efficient generation of PTH2-deficient cell models for investigation of TIP39 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PTH2 exon(s) critical for TIP39 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PTH2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TIP39 CRISPR/Cas9 KO Plasmid (h) and TIP39 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PTH2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TIP39 HDR Plasmid (h) and TIP39 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PTH2 homology arms to support homology-directed repair at defined PTH2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.