Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TIP120A Double Nickase Plasmid (h): sc-402506-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TIP120A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TIP120A Double Nickase Plasmid (h) and TIP120A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAND1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TIP120A Antibody (G-3): sc-137055
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TIP120A Double Nickase Plasmid (h) | sc-402506-NIC | 20 µg | $410.00 |
CAND1 (TIP120A) encodes a regulator of cullin-RING ubiquitin ligases that binds unneddylated cullins and promotes exchange of substrate receptor modules, thereby controlling CRL assembly and remodeling. Through this activity, TIP120A coordinates ubiquitin-dependent proteostasis and influences cell-cycle progression, DNA replication stress responses, and signal-dependent turnover of key regulatory proteins. Dysregulation of CRL dynamics and neddylation/deneddylation balance has been linked to genomic instability and altered growth control, making CAND1 a relevant node in pathways frequently perturbed in cancer biology. TIP120A function is also studied in the context of proteasome-dependent quality control and stress adaptation where substrate selection by CRLs shapes cellular phenotypes.
TIP120A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAND1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAND1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAND1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAND1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.