TIMP-2 Antibody (3A4): sc-21735

TIMP-2 Antibody (3A4) is a mouse monoclonal IgG2a kappa light chain antibody that detects TIMP-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-TIMP-2 antibody (3A4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. TIMP-2 plays a crucial role in regulating extracellular matrix remodeling by inhibiting matrix metalloproteinases, which are enzymes responsible for degrading various components of the extracellular matrix. Such regulation is vital for maintaining tissue homeostasis and is particularly important in processes such as wound healing, tissue repair, and embryonic development. Dysregulation of TIMP-2 can lead to pathological conditions, including fibrosis and cancer metastasis, highlighting TIMP-2′s significance in both normal physiology and disease states. TIMP-2 also interacts with other tissue inhibitors of metalloproteinases, such as TIMP-1, TIMP-3, and TIMP-4, to form complexes with metalloproteinases like collagenases and gelatinases, resulting in irreversible inactivation of these enzymes. This intricate balance between metalloproteinases and their inhibitors is essential for proper cellular function and tissue integrity.

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TIMP-2 Antibody (3A4) References:

1. Levels of Circulating TIMP-2 and MMP2-TIMP2 Complex Are Decreased in Squamous Cervical Carcinoma.  |  Anne, TM. and Taina, TH. 2010. Obstet Gynecol Int. 2010: 179351. PMID: 20671952
2. Primary structure and cDNA cloning of human fibroblast collagenase inhibitor.  |  Carmichael, DF., et al. 1986. Proc Natl Acad Sci U S A. 83: 2407-11. PMID: 3010309
3. The 'rejuvenating factor' TIMP-2 is detectable in human blood components for transfusion.  |  Hoefer, J., et al. 2021. Vox Sang. 116: 533-539. PMID: 33107073
4. TIMP-2 regulates 5-Fu resistance via the ERK/MAPK signaling pathway in colorectal cancer.  |  Zhang, G., et al. 2022. Aging (Albany NY). 14: 297-315. PMID: 35022331
5. TIMP-2 and IGFBP7 in human kidney biopsies in renal disease.  |  Schanz, M., et al. 2023. Clin Kidney J. 16: 1434-1446. PMID: 37664566
6. Sequence of human tissue inhibitor of metalloproteinases and its identity to erythroid-potentiating activity.  |  Docherty, AJ., et al. Nature. 318: 66-9. PMID: 3903517
7. The gene structure of tissue inhibitor of metalloproteinases (TIMP)-3 and its inhibitory activities define the distinct TIMP gene family.  |  Apte, SS., et al. 1995. J Biol Chem. 270: 14313-8. PMID: 7782289
8. Gene encoding a novel murine tissue inhibitor of metalloproteinases (TIMP), TIMP-3, is expressed in developing mouse epithelia, cartilage, and muscle, and is located on mouse chromosome 10.  |  Apte, SS., et al. 1994. Dev Dyn. 200: 177-97. PMID: 7949367
9. Cloning of cDNAs encoding human TIMP-3, a novel member of the tissue inhibitor of metalloproteinase family.  |  Silbiger, SM., et al. 1994. Gene. 141: 293-7. PMID: 8163205
10. Cloning and regulation of rat tissue inhibitor of metalloproteinases-2 in osteoblastic cells.  |  Cook, TF., et al. 1994. Arch Biochem Biophys. 311: 313-20. PMID: 8203893
11. Molecular cloning and characterization of human tissue inhibitor of metalloproteinase 4.  |  Greene, J., et al. 1996. J Biol Chem. 271: 30375-80. PMID: 8939999
12. Tissue inhibitors of metalloproteinases: structure, regulation and biological functions.  |  Gomez, DE., et al. 1997. Eur J Cell Biol. 74: 111-22. PMID: 9352216

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TIMP-2 Antibody (3A4) has 78 citations in a variety of scientific publications.

Primary antibodies like Anti-TIMP-2 Antibody (3A4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TIMP-2 Antibody (3A4) Specifications

Alternate Product Name: TIMP-2

Product Clone Name: 3A4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 56-66

Epitope Target: TIMP-2

Epitope Species: human

Product Reactivity/Detection: TIMP-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 78 citations in various scientific publications

Available Conjugates/Forms for TIMP-2 Antibody (3A4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin