Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TIMP-1 CRISPR/Cas9 KO Plasmid (h): sc-400408
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TIMP-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TIMP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TIMP-1 Antibody (G-6): sc-365905
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TIMP-1 CRISPR/Cas9 KO Plasmid (h) | sc-400408 | 20 µg | $397.00 |
Overview
TIMP1 encodes tissue inhibitor of metalloproteinases-1 (TIMP-1), a secreted glycoprotein that binds and inhibits matrix metalloproteinases to regulate extracellular matrix turnover and proteolytic signaling. By constraining MMP activity, TIMP-1 influences cell–matrix interactions, tissue remodeling, and inflammatory responses, with downstream effects on migration, invasion, and cytokine-driven remodeling programs. TIMP-1 is also implicated in MMP-independent signaling through cell-surface interactions that can modulate survival and proliferation pathways in a context-dependent manner. Altered TIMP1 expression and TIMP-1/MMP balance are frequently studied in fibrosis, arthritis, cardiovascular remodeling, and cancer biology, where dysregulated matrix dynamics contribute to disease-associated phenotypes.
TIMP-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TIMP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TIMP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TIMP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TIMP-1 protein expression.
This CRISPR knockout system enables efficient generation of TIMP1-deficient cell models for investigation of TIMP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TIMP1 exon(s) critical for TIMP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TIMP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TIMP-1 CRISPR/Cas9 KO Plasmid (h) and TIMP-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TIMP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TIMP-1 HDR Plasmid (h) and TIMP-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TIMP1 homology arms to support homology-directed repair at defined TIMP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.