Timeless Antibody (A-3): sc-393122
- Timeless Antibody (A-3) is a mouse monoclonal IgG1 κ Timeless antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of Timeless of human origin
- Timeless Antibody (A-3) is recommended for detection of Timeless of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Timeless Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-393122 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Timeless Antibody (A-3) for WB applications. These reagents are now offered in bundles with Timeless Antibody (A-3) (see ordering information below).
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SEE ALSO...
Timeless Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Timeless protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Timeless antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Timeless protein plays a crucial role in regulating circadian rhythms, which are essential for maintaining the body′s internal clock and synchronizing physiological processes with the day-night cycle. Located primarily in the nucleus, Timeless (A-3) monoclonal antibody targets a protein integral to the feedback loop that regulates core clock genes expression, including Clock and BMAL1 genes. This regulation is vital for proper circadian clock functioning, as disruptions in this system can lead to various health issues, including sleep disorders and metabolic syndromes. Timeless protein interacts with other key proteins, such as Period proteins (Per1, Per2), to modulate their activity and ensure circadian rhythm stability. Timeless (A-3) antibody enables valuable insights into molecular mechanisms underlying circadian regulation and their implications for health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Timeless Antibody (A-3) References:
- Functional identification of the mouse circadian Clock gene by transgenic BAC rescue. | Antoch, MP., et al. 1997. Cell. 89: 655-67. PMID: 9160756
- The mouse Clock mutation behaves as an antimorph and maps within the W19H deletion, distal of Kit. | King, DP., et al. 1997. Genetics. 146: 1049-60. PMID: 9215907
- Three period homologs in mammals: differential light responses in the suprachiasmatic circadian clock and oscillating transcripts outside of brain. | Zylka, MJ., et al. 1998. Neuron. 20: 1103-10. PMID: 9655499
- Mammalian circadian autoregulatory loop: a timeless ortholog and mPer1 interact and negatively regulate CLOCK-BMAL1-induced transcription. | Sangoram, AM., et al. 1998. Neuron. 21: 1101-13. PMID: 9856465
- A 24-hour circadian clock is found in the mammalian retina. | Morell, V. 1996. Science. 272: 349. PMID: 8602520
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Timeless Antibody (A-3) | sc-393122 | 200 µg/ml | $322.00 | |||
Timeless Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-527671 | 200 µg Ab; 10 µg BP | $361.00 | |||
Timeless Antibody (A-3): m-IgG1 BP-HRP Bundle | sc-533044 | 200 µg Ab; 20 µg BP | $361.00 | |||
Timeless Antibody (A-3) X | sc-393122 X | 200 µg/0.1 ml | $322.00 | |||
Timeless Antibody (A-3) AC | sc-393122 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Timeless Antibody (A-3) HRP | sc-393122 HRP | 200 µg/ml | $322.00 | |||
Timeless Antibody (A-3) FITC | sc-393122 FITC | 200 µg/ml | $336.00 | |||
Timeless Antibody (A-3) PE | sc-393122 PE | 200 µg/ml | $349.00 | |||
Timeless Antibody (A-3) Alexa Fluor® 488 | sc-393122 AF488 | 200 µg/ml | $364.00 | |||
Timeless Antibody (A-3) Alexa Fluor® 546 | sc-393122 AF546 | 200 µg/ml | $364.00 | |||
Timeless Antibody (A-3) Alexa Fluor® 594 | sc-393122 AF594 | 200 µg/ml | $364.00 | |||
Timeless Antibody (A-3) Alexa Fluor® 647 | sc-393122 AF647 | 200 µg/ml | $364.00 | |||
Timeless Antibody (A-3) Alexa Fluor® 680 | sc-393122 AF680 | 200 µg/ml | $364.00 | |||
Timeless Antibody (A-3) Alexa Fluor® 790 | sc-393122 AF790 | 200 µg/ml | $364.00 |