Tim17B Double Nickase Plasmid (h): sc-411441-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tim17B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Tim17B Double Nickase Plasmid (h) and Tim17B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TIMM17B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tim17 Antibody (H-1): sc-271152
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tim17B Double Nickase Plasmid (h) | sc-411441-NIC | 20 µg | $410.00 | |||
Tim17B Double Nickase Plasmid (h2) | sc-411441-NIC-2 | 20 µg | $410.00 |
TIMM17B encodes Tim17B, an essential component of the TIM23 translocase in the inner mitochondrial membrane that mediates import and insertion of nuclear-encoded proteins into mitochondria. By supporting mitochondrial protein homeostasis, Tim17B contributes to oxidative phosphorylation capacity, mitochondrial membrane potential maintenance, and proteostasis within the matrix and inner membrane. Perturbation of TIM23 complex function can disrupt energy metabolism and activate mitochondrial stress signaling pathways such as the mitochondrial unfolded protein response, linking TIMM17B biology to broader programs of cellular adaptation. As a mitochondrial import factor, TIMM17B is frequently examined in studies of bioenergetic reprogramming and mitochondrial dysfunction relevant to cancer cell metabolism and neurodegeneration-associated mitochondrial stress phenotypes.
Tim17B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TIMM17B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TIMM17B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TIMM17B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TIMM17B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.