Tim13A/B CRISPR/Cas9 KO Plasmid (h): sc-408855
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tim13A/B CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Tim13A/B genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tim13A/B CRISPR/Cas9 KO Plasmid (h) | sc-408855 | 20 µg | $397.00 |
Overview
TIMM13 encodes the small Tim13A/B chaperone of the mitochondrial intermembrane space that cooperates with TIMM8A/TIMM8B to escort hydrophobic precursor proteins from the TOM complex to inner membrane insertion machinery. This pathway supports oxidative phosphorylation by promoting proper biogenesis of inner membrane carriers and other multipass proteins, linking TIMM13 function to mitochondrial protein import, membrane potential maintenance, and proteostasis. Disruption of small Tim chaperone networks can contribute to mitochondrial dysfunction, elevated cellular stress responses, and impaired energy metabolism, processes frequently interrogated in neurodegeneration and cancer metabolism studies. TIMM13 is therefore relevant for research on mitochondrial quality control, respiratory chain homeostasis, and stress-adaptive signaling.
Tim13A/B CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TIMM13 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TIMM13 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TIMM13 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Tim13A/B protein expression.
This CRISPR knockout system enables efficient generation of TIMM13-deficient cell models for investigation of Tim13A/B signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TIMM13 exon(s) critical for Tim13A/B function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TIMM13 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Tim13A/B CRISPR/Cas9 KO Plasmid (h) and Tim13A/B CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TIMM13 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Tim13A/B HDR Plasmid (h) and Tim13A/B HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TIMM13 homology arms to support homology-directed repair at defined TIMM13 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.