Date published: 2026-2-14

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TIM Antibody (BB-7): sc-100541

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Datasheets
  • TIM Antibody (BB-7) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 100 µg/ml
  • raised against recombinant TIM of human origin
  • recommended for detection of TIM of mouse, rat and human origin by WB, IP, IF and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TIM Antibody (BB-7). This work is in progress.
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TIM Antibody (BB-7) is a mouse monoclonal IgG1 antibody that detects Triosephosphate Isomerase (TIM) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TIM is a crucial enzyme in the glycolytic pathway, facilitating the reversible conversion of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate, which are essential intermediates in glucose metabolism. This enzymatic activity is vital for energy production, as TIM plays a key role in the generation of pyruvate and ATP from glucose, thereby supporting cellular respiration and energy homeostasis. TIM is predominantly located in the cytosol, where TIM participates in the glycolytic process, and proper functioning is critical for maintaining metabolic balance. Mutations or deficiencies in TIM can lead to significant metabolic disorders, including non-spherocytic hemolytic anemia and other serious health issues, underscoring TIM′s importance in both normal physiology and disease states. Anti-TIM antibody (BB-7) is an invaluable tool for researchers studying glycolysis and its implications in metabolic diseases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

TIM Antibody (BB-7)

sc-100541
100 µg/ml
$339.00

I am using TIM (BB-7): sc-100541 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Exceptional immunofluorescence nuclear andExceptional immunofluorescence nuclear and cytoplasmic staining in formalin-fixed Hep G2 cells. -SCBT QC
Date published: 2015-03-04
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TIM Antibody (BB-7) is rated 5.0 out of 5 by 2.
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