TIM-3 Antibody (182H2B): sc-517632

TIM-3 Antibody (182H2B) is a mouse monoclonal IgG2b kappa light chain antibody that detects TIM-3 protein of human origin by western blotting (WB) and flow cytometry (FCM). Anti-TIM-3 antibody (182H2B) is available as a non-conjugated format, specifically designed to facilitate T cell regulation and immune response studies. TIM-3 monoclonal antibody (182H2B) enables research into TIM-3′s crucial role in modulating immune responses, particularly in T helper 1 (Th1) cells, which are essential for combating intracellular pathogens and mediating delayed-type hypersensitivity reactions. Located on Th1 cell surfaces, TIM-3 interacts with galectin-9, leading to apoptosis induction in these cells, which can significantly impact immune responses during autoimmune diseases. This interaction promotes cell death and triggers TIM-3 phosphorylation at residue Y265 by interleukin-inducible T cell kinase, Emt, highlighting post-translational modifications′ importance in regulating TIM-3′s function. TIM-3 (182H2B) antibody enables valuable insights into immune regulation mechanisms and potential therapeutic targets for autoimmune disorders and diseases characterized by dysregulated immune responses.

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TIM-3 Antibody (182H2B) References:

1. Th1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease.  |  Monney, L., et al. 2002. Nature. 415: 536-41. PMID: 11823861
2. Tim-3 inhibits T helper type 1-mediated auto- and alloimmune responses and promotes immunological tolerance.  |  Sánchez-Fueyo, A., et al. 2003. Nat Immunol. 4: 1093-101. PMID: 14556005
3. Interaction of Tim-3 and Tim-3 ligand regulates T helper type 1 responses and induction of peripheral tolerance.  |  Sabatos, CA., et al. 2003. Nat Immunol. 4: 1102-10. PMID: 14556006
4. T Cell Ig- and mucin-domain-containing molecule-3 (TIM-3) and TIM-1 molecules are differentially expressed on human Th1 and Th2 cells and in cerebrospinal fluid-derived mononuclear cells in multiple sclerosis.  |  Khademi, M., et al. 2004. J Immunol. 172: 7169-76. PMID: 15153541
5. Molecular variations in Th1-specific cell surface gene Tim-3.  |  Chae, SC., et al. 2004. Exp Mol Med. 36: 274-8. PMID: 15272240
6. Tim-3+ T-bet+ tumor-specific Th1 cells colocalize with and inhibit development and growth of murine neoplasms.  |  Simmons, WJ., et al. 2005. J Immunol. 174: 1405-15. PMID: 15661898
7. Dysregulated T cell expression of TIM3 in multiple sclerosis.  |  Koguchi, K., et al. 2006. J Exp Med. 203: 1413-8. PMID: 16754722
8. A highly conserved tyrosine of Tim-3 is phosphorylated upon stimulation by its ligand galectin-9.  |  van de Weyer, PS., et al. 2006. Biochem Biophys Res Commun. 351: 571-6. PMID: 17069754
9. TIM-3 Regulates CD103+ Dendritic Cell Function and Response to Chemotherapy in Breast Cancer.  |  de Mingo Pulido, Á., et al. 2018. Cancer Cell. 33: 60-74.e6. PMID: 29316433
10. Tim-3 regulates sepsis-induced immunosuppression by inhibiting the NF-κB signaling pathway in CD4 T cells.  |  Huang, S., et al. 2022. Mol Ther. 30: 1227-1238. PMID: 34933101
11. TIM-3 blockade in diffuse intrinsic pontine glioma models promotes tumor regression and antitumor immune memory.  |  Ausejo-Mauleon, I., et al. 2023. Cancer Cell. 41: 1911-1926.e8. PMID: 37802053