TID-1_L/S Antibody (RS-13): sc-18819

TID-1L/S Antibody (RS-13) is a mouse monoclonal IgG1 kappa light chain antibody that detects TID-1L/S protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). TID-1L/S (RS-13) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TID-1L/S promotes apoptosis, while TID-1S splice variant acts as an inhibitor of this process. The differential expression of these splice variants allows cells to respond appropriately to stress signals, influencing cell survival and death. TID-1L and TID-1S are localized to the mitochondrial matrix, where both proteins modulate apoptotic signaling pathways by affecting cytochrome c release and caspase-3 activation. Upon entering mitochondria, TID-1L and TID-1S undergo cleavage at amino acid 66, indicating mature forms derive from cytoplasmic pre-proteins. This post-translational modification highlights dynamic regulation of TID-1L/S in response to cellular conditions, underscoring significance in apoptosis and potential implications in cancer research.

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TID-1_L/S Antibody (RS-13) References:

1. TID1, a human homolog of the Drosophila tumor suppressor l(2)tid, encodes two mitochondrial modulators of apoptosis with opposing functions.  |  Syken, J., et al. 1999. Proc Natl Acad Sci U S A. 96: 8499-504. PMID: 10411904
2. Tumor suppression in Drosophila is causally related to the function of the lethal(2) tumorous imaginal discs gene, a dnaJ homolog.  |  Kurzik-Dumke, U., et al. 1995. Dev Genet. 16: 64-76. PMID: 7758246
3. The Hsp70 and Hsp60 chaperone machines.  |  Bukau, B. and Horwich, AL. 1998. Cell. 92: 351-66. PMID: 9476895
4. A novel human DnaJ protein, hTid-1, a homolog of the Drosophila tumor suppressor protein Tid56, can interact with the human papillomavirus type 16 E7 oncoprotein.  |  Schilling, B., et al. 1998. Virology. 247: 74-85. PMID: 9683573
5. Mitochondria and apoptosis.  |  Green, DR. and Reed, JC. 1998. Science. 281: 1309-12. PMID: 9721092

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TID-1_L/S Antibody (RS-13) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-TID-1_L/S Antibody (RS-13) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TID-1_L/S Antibody (RS-13) Specifications

Alternate Product Name: TID-1L/S

Product Clone Name: RS-13

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: TID-1L/S

Epitope Species: human

Product Reactivity/Detection: TID-1_L and TID-1_S

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for TID-1_L/S Antibody (RS-13): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin