Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TICAM-1 CRISPR/Cas9 KO Plasmid (h): sc-414627
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TICAM-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TICAM-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TICAM-1 Antibody (E-7): sc-514384
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TICAM-1 CRISPR/Cas9 KO Plasmid (h) | sc-414627 | 20 µg | $397.00 |
Overview
TICAM1 encodes TICAM-1 (also known as TRIF), a cytosolic adaptor that couples Toll-like receptor 3 and the TRAM-dependent branch of TLR4 signaling to innate immune transcriptional programs. Upon receptor activation, TICAM-1 coordinates assembly of signaling complexes that engage TBK1/IKKε and IRF3 to induce type I interferon responses, and it also contributes to NF-κB and MAPK pathway activation to shape inflammatory cytokine expression. This node is central to antiviral sensing of dsRNA and regulation of innate immune homeostasis in myeloid and epithelial compartments. Dysregulated TICAM-1 signaling has been implicated in altered host responses to viral infection and inflammatory phenotypes relevant to immune-mediated disease mechanisms.
TICAM-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TICAM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TICAM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TICAM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TICAM-1 protein expression.
This CRISPR knockout system enables efficient generation of TICAM1-deficient cell models for investigation of TICAM-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TICAM1 exon(s) critical for TICAM-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TICAM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TICAM-1 CRISPR/Cas9 KO Plasmid (h) and TICAM-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TICAM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TICAM-1 HDR Plasmid (h) and TICAM-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TICAM1 homology arms to support homology-directed repair at defined TICAM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.