Tiam2 Double Nickase Plasmid (h): sc-404326-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tiam2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Tiam2 Double Nickase Plasmid (h) and Tiam2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TIAM2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tiam2 Antibody (C-5): sc-514090
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tiam2 Double Nickase Plasmid (h) | sc-404326-NIC | 20 µg | $410.00 | |||
Tiam2 Double Nickase Plasmid (h2) | sc-404326-NIC-2 | 20 µg | $410.00 |
TIAM2 encodes Tiam2, a Rac-specific guanine nucleotide exchange factor (GEF) that promotes conversion of Rac1/2 to the active GTP-bound state, linking upstream receptor and adhesion cues to actin cytoskeleton remodeling. Through regulation of lamellipodia formation, cell polarity, and focal adhesion turnover, Tiam2 contributes to pathways controlling migration, invasion, and neurite outgrowth, including signaling nodes downstream of integrins, growth factor receptors, and PI3K. TIAM2 activity intersects with Rho family GTPase circuitry and MAPK-associated programs that coordinate cytoskeletal dynamics with transcriptional responses. Dysregulated Rac signaling and altered TIAM2 expression have been associated with tumor cell motility and metastatic traits in multiple cancer contexts, supporting its use as a mechanistic target in studies of cell movement and morphogenesis.
Tiam2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TIAM2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TIAM2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TIAM2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TIAM2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.