Thyroglobulin Antibody (D-9): sc-365997

Thyroglobulin Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Thyroglobulin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Thyroglobulin antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Thyroglobulin is a large preprotein that plays a crucial role in the synthesis of thyroid hormones, specifically thyroxine and triiodothyronine, which are essential for regulating metabolism, growth, and development. Located primarily in the thyroid gland, Thyroglobulin undergoes a series of post-translational modifications, including glycosylation, which are vital for stability and function. These modifications not only facilitate proper folding and dimerization but also influence secretion and interaction with other proteins in the thyroid. Importantly, defects in Thyroglobulin can lead to conditions such as goiter, characterized by an enlargement of the thyroid gland, often resulting from impaired dimerization and transport processes. Thyroglobulin monoclonal antibody (D-9) accurately detects and quantifies Thyroglobulin, making this antibody an invaluable tool for research and diagnostic applications related to thyroid function and disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Thyroglobulin Antibody (D-9) References:

1. Two novel cysteine substitutions (C1263R and C1995S) of thyroglobulin cause a defect in intracellular transport of thyroglobulin in patients with congenital goiter and the variant type of adenomatous goiter.  |  Hishinuma, A., et al. 1999. J Clin Endocrinol Metab. 84: 1438-44. PMID: 10199792
2. Expression of the Na+/I- symporter gene in human thyroid tumors: a comparison study with other thyroid-specific genes.  |  Lazar, V., et al. 1999. J Clin Endocrinol Metab. 84: 3228-34. PMID: 10487692
3. Recombinant human thyroid-stimulating hormone: pharmacology, clinical applications and potential uses.  |  Emerson, CH. and Torres, MS. 2003. BioDrugs. 17: 19-38. PMID: 12534318
4. Effect of Thyroglobulin Autoantibodies on the Metabolic Clearance of Serum Thyroglobulin.  |  Latrofa, F., et al. 2018. Thyroid. 28: 288-294. PMID: 29439614
5. Prognostic Value of Serum Thyroglobulin and Anti-Thyroglobulin Antibody in Thyroid Carcinoma Patients following Thyroidectomy.  |  Zahra, HO., et al. 2021. Diagnostics (Basel). 11: PMID: 34829426
6. Primary structure of human thyroglobulin deduced from the sequence of its 8448-base complementary DNA.  |  Malthiéry, Y. and Lissitzky, S. 1987. Eur J Biochem. 165: 491-8. PMID: 3595599
7. Thyroglobulin immunoassay with a fully automated pretreatment process provides accurate thyroglobulin values in anti-thyroglobulin antibody positive specimens.  |  Kitamura, Y., et al. 2023. Clin Biochem. 118: 110598. PMID: 37330000
8. N-glycans modulate in vivo and in vitro thyroid hormone synthesis. Study at the N-terminal domain of thyroglobulin.  |  Mallet, B., et al. 1995. J Biol Chem. 270: 29881-8. PMID: 8530385
9. Oligomeric assembly of thrombospondin in the endoplasmic reticulum of thyroid epithelial cells.  |  Prabakaran, D., et al. 1996. Eur J Cell Biol. 70: 134-41. PMID: 8793385
10. Enhanced binding to the molecular chaperone BiP slows thyroglobulin export from the endoplasmic reticulum.  |  Muresan, Z. and Arvan, P. 1998. Mol Endocrinol. 12: 458-67. PMID: 9514162
11. Demonstration of a Ca2+ requirement for thyroglobulin dimerization and export to the golgi complex.  |  Di Jeso, B., et al. 1998. Eur J Biochem. 252: 583-90. PMID: 9546677
12. Missense mutation (C1263R) in the thyroglobulin gene causes congenital goiter with mild hypothyroidism by impaired intracellular transport.  |  Hishinuma, A., et al. 1998. Endocr J. 45: 315-27. PMID: 9790265

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Thyroglobulin Antibody (D-9) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-Thyroglobulin Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Thyroglobulin Antibody (D-9) Specifications

Alternate Product Name: Thyroglobulin

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 13-41

Epitope Target: Thyroglobulin

Epitope Species: human

Product Reactivity/Detection: Thyroglobulin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for Thyroglobulin Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin