SAM Transcription Activation: SAM system utilizing dCas9-VP64, MS2-P65-HSF1, and sgRNA for precise gene transcription activation.
Thymidine Kinase CRISPR Activation Plasmid (h): sc-402954-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Thymidine Kinase CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- Thymidine Kinase CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by Thymidine Kinase CRISPR Activation Plasmid (h) and Thymidine Kinase CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the TK1 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Thymidine Kinase Antibody (C-4): sc-377211
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Thymidine Kinase CRISPR Activation Plasmid (h) | sc-402954-ACT | 20 µg | $397.00 | |||
Thymidine Kinase CRISPR Activation Plasmid (h2) | sc-402954-ACT-2 | 20 µg | $397.00 |