Thymidine Kinase Antibody (3B3.E11): sc-56967

Thymidine Kinase Antibody (3B3.E11) is a mouse monoclonal IgG1 antibody that detects Thymidine Kinase of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Thymidine Kinase antibody (3B3.E11) is available as the non-conjugated form. Thymidine Kinase (TK1) is a highly conserved phosphotransferase found in most living cells, playing a crucial role in DNA synthesis by catalyzing the phosphorylation of deoxythymidine to deoxythymidine 5′-phosphate, a key step in the nucleotide salvage pathway. This function is particularly important as TK1 facilitates the incorporation of deoxythymidine into DNA, which is essential for cell proliferation and repair. TK1 activity is significantly elevated in actively dividing cells, making TK1 a valuable biomarker for assessing cell proliferation in various clinical contexts, especially in the diagnosis and monitoring of hematological malignancies. Additionally, TK1 is critical for the efficacy of several antiviral drugs, such as azidothymidine (AZT), and is utilized in the selection of hybridoma cell lines during monoclonal antibody production. TK1 can exist in various oligomeric states, including monomers, dimers, trimers, and tetramers, which may influence activity and interactions within the cellular environment.

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Thymidine Kinase Antibody (3B3.E11) References:

1. Thymidine kinase and cancer monitoring.  |  Aufderklamm, S., et al. 2012. Cancer Lett. 316: 6-10. PMID: 22068047
2. Thymidine kinase 2 enzyme kinetics elucidate the mechanism of thymidine-induced mitochondrial DNA depletion.  |  Sun, R. and Wang, L. 2014. Biochemistry. 53: 6142-50. PMID: 25215937
3. Thymidine kinase and protein kinase in drug-resistant herpesviruses: Heads of a Lernaean Hydra.  |  Topalis, D., et al. 2018. Drug Resist Updat. 37: 1-16. PMID: 29548479
4. Thymidine kinase 1 as a tumor biomarker: technical advances offer new potential to an old biomarker.  |  Jagarlamudi, KK. and Shaw, M. 2018. Biomark Med. 12: 1035-1048. PMID: 30039979
5. Deoxynucleoside Therapy for Thymidine Kinase 2-Deficient Myopathy.  |  Domínguez-González, C., et al. 2019. Ann Neurol. 86: 293-303. PMID: 31125140
6. Thymidine Kinase-1 as Additional Diagnostic Marker of Prostate Cancer.  |  Hanousková, L., et al. 2020. Clin Lab. 66: PMID: 32538039
7. Optimization of Thymidine Kinase-Based Safety Switch for Neural Cell Therapy.  |  Locatelli, M., et al. 2022. Cells. 11: PMID: 35159311
8. Dynamics of plasma thymidine kinase activity in metastatic melanoma reflects immune checkpoint inhibitor efficacy.  |  Costa Svedman, F., et al. 2022. Acta Oncol. 61: 1116-1120. PMID: 36083107
9. Concentration of human thymidine kinase 1 discover invisible malignant human tumours.  |  Zhou, J., et al. 2022. Eur J Cell Biol. 101: 151280. PMID: 36334559
10. Therapeutic targeting at genome mutations of liver cancer by the insertion of HSV1 thymidine kinase through Cas9-mediated editing.  |  Kader, M., et al. 2024. Hepatol Commun. 8: PMID: 38497929

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Thymidine Kinase Antibody (3B3.E11) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Thymidine Kinase Antibody (3B3.E11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Thymidine Kinase Antibody (3B3.E11) Specifications

Alternate Product Name: Thymidine Kinase

Product Clone Name: 3B3.E11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Thymidine Kinase

Epitope Species: human

Product Reactivity/Detection: Thymidine Kinase

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Thymidine Kinase Antibody (3B3.E11): N/A

The preferred Thymidine Kinase antibody is Thymidine Kinase Antibody (C-4): sc-377211.