Thrombin API Antibody (D-9): sc-376933

Thrombin API Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Thrombin API protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Thrombin API antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Thrombin API (D-9) antibody recognizes a protein that plays a crucial role in the hemostatic process, particularly in converting fibrinogen to fibrin, which is essential for blood clot formation. This conversion prevents excessive bleeding following tissue injury by leading to clot stabilization and promoting platelet aggregation. Thrombin API (D-9) monoclonal antibody targets a protein involved in activating several coagulation factors, including Factors V, VII, VIII, and XIII, which further amplify the coagulation cascade. Precise regulation of Thrombin activity is critical, as dysregulation can lead to thrombotic disorders or excessive bleeding. Understanding Thrombin API function and interactions within the coagulation cascade is essential for developing therapeutic strategies for various cardiovascular diseases and conditions related to hemostasis.

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Thrombin API Antibody (D-9) References:

1. Thrombin inhibits tumor cell growth in association with up-regulation of p21(waf/cip1) and caspases via a p53-independent, STAT-1-dependent pathway.  |  Huang, YQ., et al. 2000. J Biol Chem. 275: 6462-8. PMID: 10692450
2. Effect of oligodeoxynucleotide thrombin aptamer on thrombin inhibition by heparin cofactor II and antithrombin.  |  Holland, CA., et al. 2000. FEBS Lett. 484: 87-91. PMID: 11068038
3. Activation of thromboplastinogen by thrombin.  |  QUICK, AJ., et al. 1953. Am J Physiol. 174: 123-6. PMID: 13065506
4. Interaction of thrombin and fibrinogen.  |  LORAND, L. 1954. Physiol Rev. 34: 742-52. PMID: 13215096
5. Venous stasis and thrombin generation.  |  Colucci, G., et al. 2004. J Thromb Haemost. 2: 1008-9. PMID: 15140142
6. The coagulation cascade: initiation, maintenance, and regulation.  |  Davie, EW., et al. 1991. Biochemistry. 30: 10363-70. PMID: 1931959
7. An unexpected link between angiotensinogen and thrombin.  |  Wang, Y. and Ragg, H. 2011. FEBS Lett. 585: 2395-9. PMID: 21722639
8. Basic mechanisms in blood coagulation.  |  Davie, EW. and Fujikawa, K. 1975. Annu Rev Biochem. 44: 799-829. PMID: 237463
9. Proteolytic derivatives of thrombin.  |  Elion, J., et al. 1986. Ann N Y Acad Sci. 485: 16-26. PMID: 2436522
10. Human genes encoding prothrombin and ceruloplasmin map to 11p11-q12 and 3q21-24, respectively.  |  Royle, NJ., et al. 1987. Somat Cell Mol Genet. 13: 285-92. PMID: 3474786
11. Actions of thrombin and other coagulant and proteolytic enzymes on blood platelets.  |  Davey, MG. and Lüscher, EF. 1967. Nature. 216: 857-8. PMID: 6074962
12. Thrombin stimulates fibroblast procollagen production via proteolytic activation of protease-activated receptor 1.  |  Chambers, RC., et al. 1998. Biochem J. 333 (Pt 1): 121-7. PMID: 9639571

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Thrombin API Antibody (D-9) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Thrombin API Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Thrombin API Antibody (D-9) Specifications

Alternate Product Name: Thrombin Activation Peptide 1

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 71-155

Epitope Target: Thrombin Activation Peptide 1

Epitope Species: human

Product Reactivity/Detection: Thrombin API and Prothrombin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Thrombin API Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Thrombin antibody is Thrombin Antibody (F-1): sc-271449.