THP Antibody (B-2): sc-271022

THP Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects THP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). THP Antibody (B-2) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tamm-Horsfall glycoprotein (THP), also known as uromodulin, is the most abundant protein found in normal urine, synthesized by kidney epithelial cells and excreted at a rate of 50-100 mg per day. THP plays a crucial role in the urinary system, particularly in maintaining renal water balance and modulating immune responses. THP is involved in the formation of calcium-containing kidney stones, alongside other urinary glycoproteins such as uropontin and nephrocalcin. A unique glycosyl phosphatidylinositol (GPI) anchor allows THP to be expressed on the luminal surface of renal epithelial cells, where THP can effectively inhibit bacterial adhesion and block early events required for T-cell proliferation, highlighting THP′s potential as a therapeutic target in immunological disorders. The gene encoding THP is located on human chromosome 16p12.3 and is considered a candidate gene for nephrolithiasis, making THP a significant focus for research in kidney health and disease.

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THP Antibody (B-2) References:

1. Analysis of the C-terminal structure of urinary Tamm-Horsfall protein reveals that the release of the glycosyl phosphatidylinositol-anchored counterpart from the kidney occurs by phenylalanine-specific proteolysis.  |  Fukuoka, S. and Kobayashi, K. 2001. Biochem Biophys Res Commun. 289: 1044-8. PMID: 11741296
2. Isolation of mouse THP gene promoter and demonstration of its kidney-specific activity in transgenic mice.  |  Zhu, X., et al. 2002. Am J Physiol Renal Physiol. 282: F608-17. PMID: 11880321
3. Tamm-Horsfall glycoprotein: biology and clinical relevance.  |  Serafini-Cessi, F., et al. 2003. Am J Kidney Dis. 42: 658-76. PMID: 14520616
4. A mucoprotein derived from human urine which reacts with influenza, mumps, and Newcastle disease viruses.  |  TAMM, I. and HORSFALL, FL. 1952. J Exp Med. 95: 71-97. PMID: 14907962
5. N-Glycans carried by Tamm-Horsfall glycoprotein have a crucial role in the defense against urinary tract diseases.  |  Serafini-Cessi, F., et al. 2005. Glycoconj J. 22: 383-94. PMID: 16622944
6. Uromodulin: a unique 85-kilodalton immunosuppressive glycoprotein isolated from urine of pregnant women.  |  Muchmore, AV. and Decker, JM. 1985. Science. 229: 479-81. PMID: 2409603
7. Immunomodulatory properties of Tamm-Horsfall glycoprotein (THP) and uromodulin.  |  Hong, CY., et al. 2015. Asian Pac J Allergy Immunol. 33: 26-32. PMID: 25840631
8. Tamm Horsfall Glycoprotein and Uromodulin: It Is All about the Tubules!  |  Bleyer, AJ. and Kmoch, S. 2016. Clin J Am Soc Nephrol. 11: 6-8. PMID: 26683889
9. Tamm-Horsfall glycoprotein engages human Siglec-9 to modulate neutrophil activation in the urinary tract.  |  Patras, KA., et al. 2017. Immunol Cell Biol. 95: 960-965. PMID: 28829050
10. Optimization of differentiation and transcriptomic profile of THP-1 cells into macrophage by PMA.  |  Liu, T., et al. 2023. PLoS One. 18: e0286056. PMID: 37459313
11. Localization of the Tamm-Horsfall glycoprotein (uromodulin) gene to chromosome 16p12.3-16p13.11.  |  Pook, MA., et al. 1993. Ann Hum Genet. 57: 285-90. PMID: 8179291
12. Chromosomal assignment of the uromodulin gene (UMOD) to 16p13.11.  |  Jeanpierre, C., et al. 1993. Cytogenet Cell Genet. 62: 185-7. PMID: 8382593

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-THP Antibody (B-2) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-THP Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

THP Antibody (B-2) Specifications

Alternate Product Name: Uromodulin

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 291-425

Epitope Target: THP

Epitope Species: human

Product Reactivity/Detection: THP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for THP Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin