THADA CRISPR/Cas9 KO Plasmid (h): sc-405868
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- THADA CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the THADA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
THADA CRISPR/Cas9 KO Plasmid (h) | sc-405868 | 20 µg | $397.00 |
Overview
THADA (thyroid adenoma associated) encodes a large cytoplasmic protein implicated in cellular energy homeostasis and stress-adaptive processes, with reported links to endoplasmic reticulum function and calcium handling. Genetic studies associate THADA with thyroid neoplasia susceptibility and endocrine-related traits, and altered THADA activity has been connected to metabolic phenotypes across populations. In cell models, THADA perturbation is used to interrogate pathways that couple nutrient sensing, organelle physiology, and cell growth control. These features make THADA a useful target for mechanistic studies of metabolism-associated signaling and context-specific tumor biology.
THADA CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the THADA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the THADA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the THADA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish THADA protein expression.
This CRISPR knockout system enables efficient generation of THADA-deficient cell models for investigation of THADA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting THADA exon(s) critical for THADA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple THADA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by THADA CRISPR/Cas9 KO Plasmid (h) and THADA CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the THADA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by THADA HDR Plasmid (h) and THADA HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by THADA homology arms to support homology-directed repair at defined THADA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.