TGN38 Antibody (G-9): sc-271624

TGN38 Antibody (G-9) is a mouse monoclonal IgG1 antibody that detects TGN38 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TGN38, also known as trans-Golgi network protein 2, is a type I integral membrane protein that plays a crucial role in the sorting and transport of proteins within the cell. TGN38 is primarily located in the trans-Golgi network and the plasma membrane, where TGN38 facilitates the partitioning of nascent proteins into carrier vesicles destined for their appropriate cellular locations. This localization is vital for maintaining cellular homeostasis and ensuring that proteins reach their functional sites, which is essential for various cellular processes, including secretion and membrane trafficking. The cytosolic domain of TGN38 interacts with AP2 Clathrin adaptor complexes through a specific tyrosine-containing motif, enabling the internalization of proteins from the plasma membrane. Additionally, TGN38 undergoes post-translational modifications, including N- and O-linked glycosylation, which are important for stability, localization, and function. These modifications contribute to TGN38′s presence in key tissues such as the brain, lung, and kidney, highlighting TGN38′s significance in both normal physiology and potential pathophysiological conditions. Anti-TGN38 antibody (G-9) is an invaluable tool for researchers studying protein transport mechanisms and the role of the trans-Golgi network in cellular function.

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TGN38 Antibody (G-9) References:

1. Chimeric forms of furin and TGN38 are transported with the plasma membrane in the trans-Golgi network via distinct endosomal pathways.  |  Mallet, WG. and Maxfield, FR. 1999. J Cell Biol. 146: 345-59. PMID: 10465644
2. GLUT4 recycles via a trans-Golgi network (TGN) subdomain enriched in Syntaxins 6 and 16 but not TGN38: involvement of an acidic targeting motif.  |  Shewan, AM., et al. 2003. Mol Biol Cell. 14: 973-86. PMID: 12631717
3. Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).  |  Luzio, JP., et al. 1990. Biochem J. 270: 97-102. PMID: 2204342
4. Analysis of the co-localization of the insulin-responsive glucose transporter (GLUT4) and the trans Golgi network marker TGN38 within 3T3-L1 adipocytes.  |  Martin, S., et al. 1994. Biochem J. 300 (Pt 3): 743-9. PMID: 8010955
5. The tyrosine-containing internalization motif in the cytoplasmic domain of TGN38/41 lies within a nascent helix.  |  Wilde, A., et al. 1994. J Biol Chem. 269: 7131-6. PMID: 8125922
6. An electron microscopic study of TGN38/41 dynamics.  |  Ladinsky, MS. and Howell, KE. 1993. J Cell Sci Suppl. 17: 41-7. PMID: 8144704
7. A cytosolic complex of p62 and rab6 associates with TGN38/41 and is involved in budding of exocytic vesicles from the trans-Golgi network.  |  Jones, SM., et al. 1993. J Cell Biol. 122: 775-88. PMID: 8349729
8. Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence.  |  Humphrey, JS., et al. 1993. J Cell Biol. 120: 1123-35. PMID: 8436587
9. TGN38 is maintained in the trans-Golgi network by a tyrosine-containing motif in the cytoplasmic domain.  |  Bos, K., et al. 1993. EMBO J. 12: 2219-28. PMID: 8491209
10. Serine 331 and tyrosine 333 are both involved in the interaction between the cytosolic domain of TGN38 and the mu2 subunit of the AP2 clathrin adaptor complex.  |  Stephens, DJ., et al. 1997. J Biol Chem. 272: 14104-9. PMID: 9162036

The preferred TGN38 antibody is TGN38 Antibody (B-6): sc-166594.