TGase1 Antibody (E-6): sc-166467

TGase1 Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects TGase1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). TGase1 (E-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TGase1, also known as transglutaminase K, plays a crucial role in forming the cross-linked cell envelope (CE) in terminally differentiating mammalian epidermal cells, which is essential for maintaining skin integrity and barrier function. The CE, a 10 to 20 nm thick protein deposit located on the intracellular surface of the plasma membrane, is generated through the action of transglutaminases, which catalyze the formation of isopeptide bonds by cross-linking glutamine and lysine residues in substrate proteins. This process is vital for various biological functions, including epidermal differentiation and the regulation of cell adhesion, which ultimately influences skin health and resilience. TGase1 is a membrane-associated protein consisting of 817 amino acids, and calcium ions regulate TGase1 activity, highlighting TGase1′s importance in cellular signaling and structural integrity. Additionally, TGase1 interacts with other proteins involved in skin barrier formation, further underscoring TGase1′s significance in dermatological research and potential therapeutic applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TGase1 Antibody (E-6) References:

1. A novel function for transglutaminase 1: attachment of long-chain omega-hydroxyceramides to involucrin by ester bond formation.  |  Nemes, Z., et al. 1999. Proc Natl Acad Sci U S A. 96: 8402-7. PMID: 10411887
2. Regulation of transglutaminases by nitric oxide.  |  Bernassola, F., et al. 1999. Ann N Y Acad Sci. 887: 83-91. PMID: 10668466
3. Structure and organization of the human transglutaminase 1 gene.  |  Kim, IG., et al. 1992. J Biol Chem. 267: 7710-7. PMID: 1348508
4. Isolation and characterization of cDNA clones to mouse macrophage and human endothelial cell tissue transglutaminases.  |  Gentile, V., et al. 1991. J Biol Chem. 266: 478-83. PMID: 1670766
5. Molecular cloning of human epidermal transglutaminase cDNA from keratinocytes in culture.  |  Yamanishi, K., et al. 1991. Biochem Biophys Res Commun. 175: 906-13. PMID: 1673840
6. Transglutaminase-1 regulates renal epithelial cell proliferation through activation of Stat-3.  |  Zhang, Z., et al. 2009. J Biol Chem. 284: 3345-3353. PMID: 19049964
7. Transglutaminase-1 gene mutations in autosomal recessive congenital ichthyosis: summary of mutations (including 23 novel) and modeling of TGase-1.  |  Herman, ML., et al. 2009. Hum Mutat. 30: 537-47. PMID: 19241467
8. In situ detection of active transglutaminases for keratinocyte type (TGase 1) and tissue type (TGase 2) using fluorescence-labeled highly reactive substrate peptides.  |  Itoh, M., et al. 2011. J Histochem Cytochem. 59: 180-7. PMID: 20876521
9. The UVB-Stimulated Expression of Transglutaminase 1 Is Mediated Predominantly via the NFκB Signaling Pathway: New Evidence of Its Significant Attenuation through the Specific Interruption of the p38/MSK1/NFκBp65 Ser276 Axis.  |  Terazawa, S., et al. 2015. PLoS One. 10: e0136311. PMID: 26305102
10. Deep Association between Transglutaminase 1 and Tissue Eosinophil Infiltration Leading to Nasal Polyp Formation and/or Maintenance with Fibrin Polymerization in Chronic Rhinosinusitis with Nasal Polyps.  |  Sonoyama, T., et al. 2022. Int J Mol Sci. 23: PMID: 36361742
11. A pan-cancer analysis of the oncogenic and immunological roles of transglutaminase 1 (TGM1) in human cancer.  |  Wu, R., et al. 2024. J Cancer Res Clin Oncol. 150: 123. PMID: 38472489
12. Dual functions of transglutaminase in novel cell adhesion.  |  Ueki, S., et al. 1996. J Cell Sci. 109 (Pt 11): 2727-35. PMID: 8937990

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TGase1 Antibody (E-6) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-TGase1 Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TGase1 Antibody (E-6) Specifications

Alternate Product Name: TGase1

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 3-22

Epitope Target: TGase1

Epitope Species: human

Product Reactivity/Detection: TGase1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for TGase1 Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin