TFPI Antibody (G-5): sc-365920

TFPI Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects TFPI protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TFPI (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The tissue factor pathway inhibitor (TFPI) plays a crucial role in regulating the extrinsic pathway of blood coagulation, which is vital for maintaining hemostasis and preventing excessive bleeding. TFPI functions by directly inhibiting factor Xa and, in an Xa-dependent manner, also inhibits the catalytic complex formed by factor VIIa and tissue factor, thereby preventing the propagation of coagulation. This regulation is particularly important in pathological conditions where tissue factor is aberrantly expressed, such as in cancer and inflammatory diseases, as TFPI helps to control thrombus formation and maintain vascular integrity. Additionally, TFPI is a multivalent, Kunitz-type proteinase inhibitor that circulates in association with plasma lipoproteins, including very low-density lipoprotein, low-density lipoprotein, and high-density lipoprotein, which may influence bioavailability and functional activity in the bloodstream. TFPI-2, a related glycoprotein originally isolated from human placenta, further underscores the complexity and significance of the TFPI family in coagulation and cellular signaling processes.

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TFPI Antibody (G-5) References:

1. The coagulation cascade: initiation, maintenance, and regulation.  |  Davie, EW., et al. 1991. Biochemistry. 30: 10363-70. PMID: 1931959
2. Structure of the human lipoprotein-associated coagulation inhibitor gene. Intro/exon gene organization and localization of the gene to chromosome 2.  |  Girard, TJ., et al. 1991. J Biol Chem. 266: 5036-41. PMID: 2002045
3. The Kunitz-3 domain of TFPI-alpha is required for protein S-dependent enhancement of factor Xa inhibition.  |  Ndonwi, M., et al. 2010. Blood. 116: 1344-51. PMID: 20479289
4. Syndecan-3 and TFPI colocalize on the surface of endothelial-, smooth muscle-, and cancer cells.  |  Tinholt, M., et al. 2015. PLoS One. 10: e0117404. PMID: 25617766
5. Antibodies against TFPI and protein C are associated with a severe thrombotic phenotype in patients with and without antiphospholipid syndrome.  |  Efthymiou, M., et al. 2018. Thromb Res. 170: 60-68. PMID: 30121005
6. Characterization of the inhibition of tissue factor in serum.  |  Broze, GJ. and Miletich, JP. 1987. Blood. 69: 150-5. PMID: 3024756
7. Levels of TAFI, TFPI and ADAMTS-13 in inflammatory bowel disease.  |  Yüzbaşıoğlu, B., et al. 2019. Turk J Gastroenterol. 30: 1025-1029. PMID: 31854307
8. Studies of a mechanism inhibiting the initiation of the extrinsic pathway of coagulation.  |  Rao, LV. and Rapaport, SI. 1987. Blood. 69: 645-51. PMID: 3492226
9. Elevated TFPI is a prognostic factor in hepatocellular carcinoma: Putative role of miR-7-5p and miR-1236-3p.  |  Sletten, M., et al. 2024. Thromb Res. 241: 109073. PMID: 38945092
10. Evidence that a second human tissue factor pathway inhibitor (TFPI-2) and human placental protein 5 are equivalent.  |  Kisiel, W., et al. 1994. Blood. 84: 4384-5. PMID: 7994054
11. Human tissue factor pathway inhibitor (TFPI) gene: complete genomic structure and localization on the genetic map of chromosome 2q.  |  Enjyoji, K., et al. 1993. Genomics. 17: 423-8. PMID: 8104873
12. Assignment of the human PP5/TFPI-2 gene to 7q22 by FISH and PCR-based human/rodent cell hybrid mapping panel analysis.  |  Miyagi, Y., et al. 1996. Genomics. 35: 267-8. PMID: 8661135

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFPI Antibody (G-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-TFPI Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFPI Antibody (G-5) Specifications

Alternate Product Name: TFPI

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 27-146

Epitope Target: TFPI

Epitope Species: mouse

Product Reactivity/Detection: precursor and mature forms of TFPI

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for TFPI Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin