TFIIH p80 Antibody (184.7)recommended for detection of TFIIH p80 of mouse, rat and human origin by WB, IP and ELISA

TFIIH p80 Antibody (184.7): sc-101174

TFIIH p80 Antibody (184.7) is rated 4.0 out of 5 by 4.
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Datasheets

    • TFIIH p80 Antibody (184.7) is a mouse monoclonal IgG1 (kappa light chain) provided at 100 µg/ml
    • raised against recombinant TFIIH p80 of human origin
    • recommended for detection of TFIIH p80 of mouse, rat and human origin by WB, IP and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I will use this antibody to detect a truncated version of ERCC2, therefore I would like to know the epitope that is recognized by this antibody. Could you please tell me where exactly the antibody binds? Thanks a lot.

Asked by: PostDoc
Thank you for your question. This antibody was made against a full length recombinant protein. The exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2019-01-14

For Western Blot, is it recommended to use denatured or non-denatured conditions with TFIIH p80 (184.7): sc-101174 antibody?

Asked by: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 3 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-03-29
Rated 4 out of 5 by from Published IP data using yeast samples Published IP data using yeast samples. -SCBT Publication Review
Date published: 2015-03-22
Rated 4 out of 5 by from Successfully used to IP TFIIH p80 from HeLa Successfully used to IP TFIIH p80 from HeLa cells; verified pull down via WB with the same antibody. -SCBT Publication Review
Date published: 2015-01-09
Rated 5 out of 5 by from Clean band in western blot analysis of TFIIH Clean band in western blot analysis of TFIIH expression in HeLa nuclear extract. -SCBT QC
Date published: 2013-03-11
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