TFIIH p52 Antibody (A-10): sc-514448

TFIIH p52 Antibody (A-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects TFIIH p52 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TFIIH p52 (A-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIH p52 plays a crucial role in transcription and DNA repair mechanisms, functioning as part of the TFIIH complex, which is essential for unwinding DNA during transcription initiation and nucleotide excision repair. The TFIIH complex is composed of several subunits, including p89, p62, p52, p44, and p34, with p89 and p80 exhibiting ATP-dependent helicase activity, which is vital for separating DNA strands. The p62, p52, and p44 subunits are particularly important for nucleotide excision repair, a critical cellular process that removes DNA lesions caused by environmental factors such as UV radiation. By facilitating the repair of damaged DNA, TFIIH p52 helps maintain genomic stability, making TFIIH p52 an important target for studies related to cancer and other diseases associated with DNA damage.

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TFIIH p52 Antibody (A-10) References:

1. Cloning of the 62-kilodalton component of basic transcription factor BTF2.  |  Fischer, L., et al. 1992. Science. 257: 1392-5. PMID: 1529339
2. Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH.  |  Flores, O., et al. 1992. J Biol Chem. 267: 2786-93. PMID: 1733973
3. Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2.  |  Gerard, M., et al. 1991. J Biol Chem. 266: 20940-5. PMID: 1939143
4. A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome.  |  Weeda, G., et al. 1990. Cell. 62: 777-91. PMID: 2167179
5. ERCC2: cDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3.  |  Weber, CA., et al. 1990. EMBO J. 9: 1437-47. PMID: 2184031
6. An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters.  |  Conaway, RC. and Conaway, JW. 1989. Proc Natl Acad Sci U S A. 86: 7356-60. PMID: 2552440
7. Structure and predictive functional profiling of microbial communities in two biotrickling filters treated with continuous/discontinuous waste gases.  |  Feng, R., et al. 2019. AMB Express. 9: 2. PMID: 30610394
8. Enhancement of using combined packing materials on the removal of mixed sulfur compounds in a biotrickling filter and analysis of microbial communities.  |  Tu, X., et al. 2019. BMC Biotechnol. 19: 52. PMID: 31345193
9. Structural and dynamical insights into the PH domain of p62 in human TFIIH.  |  Okuda, M., et al. 2021. Nucleic Acids Res. 49: 2916-2930. PMID: 33211877
10. Effect of rhamnolipids on the biodegradation of m-dichlorobenzene in biotrickling filters: Performance and mechanism.  |  Yang, B., et al. 2022. J Environ Manage. 320: 115951. PMID: 36056502
11. p44 and p34 subunits of the BTF2/TFIIH transcription factor have homologies with SSL1, a yeast protein involved in DNA repair.  |  Humbert, S., et al. 1994. EMBO J. 13: 2393-8. PMID: 8194529
12. Cloning and characterization of p52, the fifth subunit of the core of the transcription/DNA repair factor TFIIH.  |  Marinoni, JC., et al. 1997. EMBO J. 16: 1093-102. PMID: 9118947

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFIIH p52 Antibody (A-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-TFIIH p52 Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFIIH p52 Antibody (A-10) Specifications

Alternate Product Name: TFIIH p52

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 20-319

Epitope Target: TFIIH p52

Epitope Species: human

Product Reactivity/Detection: TFIIH p52

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for TFIIH p52 Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin