TFIIF RAP 74 CRISPR/Cas9 KO Plasmid (h): sc-405237
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TFIIF RAP 74 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TFIIF RAP 74 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIF RAP 74 CRISPR/Cas9 KO Plasmid (h) | sc-405237 | 20 µg | $397.00 |
Overview
GTF2F1 encodes the 74 kDa subunit of transcription factor IIF (TFIIF), also known as TFIIF RAP 74, a core component of the RNA polymerase II general transcription machinery. TFIIF stabilizes the preinitiation complex with TFIIB and TFIIE, supports promoter clearance, and modulates transcription elongation while helping coordinate transcription with co-transcriptional RNA processing. Through these activities, GTF2F1 contributes to global gene expression control, cell-cycle progression, and responses to replication or transcription stress. Altered regulation of general transcription factors and Pol II dynamics is frequently associated with dysregulated proliferative programs and genome instability, making GTF2F1 a useful node for mechanistic studies in cancer biology and transcription-linked disease phenotypes.
TFIIF RAP 74 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GTF2F1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GTF2F1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GTF2F1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TFIIF RAP 74 protein expression.
This CRISPR knockout system enables efficient generation of GTF2F1-deficient cell models for investigation of TFIIF RAP 74 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GTF2F1 exon(s) critical for TFIIF RAP 74 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GTF2F1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TFIIF RAP 74 CRISPR/Cas9 KO Plasmid (h) and TFIIF RAP 74 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GTF2F1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TFIIF RAP 74 HDR Plasmid (h) and TFIIF RAP 74 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GTF2F1 homology arms to support homology-directed repair at defined GTF2F1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.