TFIIF RAP 30 Antibody (G-3): sc-374304
- TFIIF RAP 30 Antibody (G-3) is a mouse monoclonal IgG1 κ TFIIF RAP 30 antibody provided at 200 µg/ml
- raised against amino acids 102-249 mapping near the C-terminus of TFIIF RAP 30 of human origin
- TFIIF RAP 30 Antibody (G-3) is recommended for detection of TFIIF RAP 30 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-TFIIF RAP 30 Antibody (G-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-374304 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TFIIF RAP 30 Antibody (G-3) for WB applications. These reagents are now offered in bundles with TFIIF RAP 30 Antibody (G-3) (see ordering information below).
TFIIF RAP 30 Antibody (G-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects TFIIF RAP 30 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-TFIIF RAP 30 antibody (G-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIF RAP 30 (G-3) antibody recognizes a protein that plays a crucial role in the transcription process, specifically as part of the TFIIF complex, which is essential for the assembly of RNA polymerase II into the preinitiation complex at the promoter region of protein-coding genes. This complex formation is vital for accurate initiation of transcription, as TFIIF not only aids in RNA polymerase II recruitment but also enhances transcription elongation. TFIIF RAP 30, along with RAP 74, ensures efficient operation of the transcription machinery, which is critical for proper gene expression and cellular function. Disruptions in this process can lead to various diseases, highlighting the significance of studying TFIIF RAP 30 and its interactions within the transcriptional machinery.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TFIIF RAP 30 Antibody (G-3) References:
- TFIIA induces conformational changes in TFIID via interactions with the basic repeat. | Lee, DK., et al. 1992. Mol Cell Biol. 12: 5189-96. PMID: 1406690
- Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF. | Aso, T., et al. 1992. Nature. 355: 461-4. PMID: 1734283
- Structure and functional properties of human general transcription factor IIE. | Peterson, MG., et al. 1991. Nature. 354: 369-73. PMID: 1956398
- Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex. | Maldonado, E., et al. 1990. Mol Cell Biol. 10: 6335-47. PMID: 2247058
- Functional domains and upstream activation properties of cloned human TATA binding protein. | Peterson, MG., et al. 1990. Science. 248: 1625-30. PMID: 2363050
- Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II. | Sopta, M., et al. 1989. Nature. 341: 410-4. PMID: 2477704
- Domain structure of a human general transcription initiation factor, TFIIF. | Yonaha, M., et al. 1993. Nucleic Acids Res. 21: 273-9. PMID: 8441635
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIF RAP 30 Antibody (G-3) | sc-374304 | 200 µg/ml | $322.00 | |||
TFIIF RAP 30 Antibody (G-3): m-IgG Fc BP-HRP Bundle | sc-527450 | 200 µg Ab; 10 µg BP | $361.00 | |||
TFIIF RAP 30 Antibody (G-3): m-IgG1 BP-HRP Bundle | sc-532823 | 200 µg Ab; 20 µg BP | $361.00 | |||
TFIIF RAP 30 Antibody (G-3) X | sc-374304 X | 200 µg/0.1 ml | $322.00 | |||
TFIIF RAP 30 Antibody (G-3) AC | sc-374304 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TFIIF RAP 30 Antibody (G-3) HRP | sc-374304 HRP | 200 µg/ml | $322.00 | |||
TFIIF RAP 30 Antibody (G-3) FITC | sc-374304 FITC | 200 µg/ml | $336.00 | |||
TFIIF RAP 30 Antibody (G-3) PE | sc-374304 PE | 200 µg/ml | $349.00 | |||
TFIIF RAP 30 Antibody (G-3) Alexa Fluor® 488 | sc-374304 AF488 | 200 µg/ml | $364.00 | |||
TFIIF RAP 30 Antibody (G-3) Alexa Fluor® 546 | sc-374304 AF546 | 200 µg/ml | $364.00 | |||
TFIIF RAP 30 Antibody (G-3) Alexa Fluor® 594 | sc-374304 AF594 | 200 µg/ml | $364.00 | |||
TFIIF RAP 30 Antibody (G-3) Alexa Fluor® 647 | sc-374304 AF647 | 200 µg/ml | $364.00 | |||
TFIIF RAP 30 Antibody (G-3) Alexa Fluor® 680 | sc-374304 AF680 | 200 µg/ml | $364.00 | |||
TFIIF RAP 30 Antibody (G-3) Alexa Fluor® 790 | sc-374304 AF790 | 200 µg/ml | $364.00 |