TFIIB Antibody (24): sc-136190
- TFIIB Antibody (24) is a mouse monoclonal IgG2a, cited in 1 publications, provided at 50 µg/0.5 ml
- raised against amino acids 2-155 of TFIIB of human origin
- recommended for detection of TFIIB of mouse, rat, human and canine origin by WB, IP and IF
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TFIIB Antibody (24). This work is in progress.
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TFIIB Antibody (24) is a mouse monoclonal IgG2a antibody that detects TFIIB in mouse, rat, human, and canine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). TFIIB plays a crucial role in the transcription initiation process by serving as a bridge between the TATA-binding protein (TBP) and RNA polymerase II, facilitating the assembly of the transcription pre-initiation complex. This interaction is vital for the accurate transcription of protein-coding genes, as TFIIB ensures RNA polymerase II is properly positioned at the promoter region, allowing for the correct initiation of transcription. TFIIB′s function extends beyond structural support, influencing the efficiency and regulation of gene expression, making TFIIB a key player in cellular processes such as development, differentiation, and response to environmental signals. Through interactions with various transcription factors and components of the transcription machinery, TFIIB maintains the fidelity of gene expression, which is critical for normal cellular function and organismal development. Anti-TFIIB antibody (24) serves as an invaluable tool for researchers studying transcription regulation and its implications in various biological contexts.
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TFIIB Antibody (24) References:
- A conformational change in TFIIB is required for activator-mediated assembly of the preinitiation complex. | Glossop, JA., et al. 2004. Nucleic Acids Res. 32: 1829-35. PMID: 15037660
- A TFIIB-like protein is indispensable for spliced leader RNA gene transcription in Trypanosoma brucei. | Schimanski, B., et al. 2006. Nucleic Acids Res. 34: 1676-84. PMID: 16554554
- Localization of TFIIB binding regions using serial analysis of chromatin occupancy. | Yochum, GS., et al. 2007. BMC Mol Biol. 8: 102. PMID: 17997859
- TAF1B is a TFIIB-like component of the basal transcription machinery for RNA polymerase I. | Naidu, S., et al. 2011. Science. 333: 1640-2. PMID: 21921199
- Structure and function of the initially transcribing RNA polymerase II-TFIIB complex. | Sainsbury, S., et al. 2013. Nature. 493: 437-40. PMID: 23151482
- The σ enigma: bacterial σ factors, archaeal TFB and eukaryotic TFIIB are homologs. | Burton, SP. and Burton, ZF. 2014. Transcription. 5: e967599. PMID: 25483602
- Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2. | Gouge, J., et al. 2015. Cell. 163: 1375-87. PMID: 26638071
- Release of Human TFIIB from Actively Transcribing Complexes Is Triggered upon Synthesis of 7- and 9-nt RNAs. | Ly, E., et al. 2020. J Mol Biol. 432: 4049-4060. PMID: 32417370
- Differential dependencies of human RNA polymerase II promoters on TBP, TAF1, TFIIB and XPB. | Santana, JF., et al. 2022. Nucleic Acids Res. 50: 9127-9148. PMID: 35947745
- Glycosylation analysis of transcription factor TFIIB using bioinformatics and experimental methods. | Uslupehlivan, M. and Deveci, R. 2024. J Biomol Struct Dyn. 1-11. PMID: 39601751
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIB Antibody (24) | sc-136190 | 50 µg/0.5 ml | $322.00 |