TFIIA-γ Antibody (D-6): sc-374483
- TFIIA-γ Antibody (D-6) is a mouse monoclonal IgG1 κ TFIIA-γ antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 2-29 at the N-terminus of TFIIA-γ of human origin
- TFIIA-gamma Antibody (D-6) is recommended for detection of TFIIA-γ of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- TransCruz reagent for ChIP application (sc-374483 X, 200 µg/0.1 ml)
- Anti-TFIIA-gamma Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TFIIA-γ Antibody (D-6) for WB applications. These reagents are now offered in bundles with TFIIA-γ Antibody (D-6) (see ordering information below).
TFIIA-γ Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TFIIA-gamma protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TFIIA-γ (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. TFIIA-gamma protein plays a crucial role in initiating transcription from protein-coding genes in eukaryotes, a process essential for gene expression and cellular function. TFIIA-gamma functions within a multiprotein complex including several basal transcription factors, with TFIIA-gamma interaction being vital for proper assembly of transcription machinery. TFIIA consists of three subunits: TFIIA-α, TFIIA-β, and TFIIA-γ, which work together to enhance TATA-binding protein (TBP) binding to the TATA box, a key element in gene promoter regions. TFIIA-gamma′s regulation of TBP ensures accurate and efficient transcription initiation, influencing gene expression patterns fundamental to various biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TFIIA-γ Antibody (D-6) References:
- Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH. | Flores, O., et al. 1992. J Biol Chem. 267: 2786-93. PMID: 1733973
- Purification and interaction properties of the human RNA polymerase B(II) general transcription factor BTF2. | Gerard, M., et al. 1991. J Biol Chem. 266: 20940-5. PMID: 1939143
- Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex. | Maldonado, E., et al. 1990. Mol Cell Biol. 10: 6335-47. PMID: 2247058
- An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters. | Conaway, RC. and Conaway, JW. 1989. Proc Natl Acad Sci U S A. 86: 7356-60. PMID: 2552440
- Five intermediate complexes in transcription initiation by RNA polymerase II. | Buratowski, S., et al. 1989. Cell. 56: 549-61. PMID: 2917366
- Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID. | Nakajima, N., et al. 1988. Mol Cell Biol. 8: 4028-40. PMID: 3185540
- Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription. | DeJong, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 3313-7. PMID: 7724559
- Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription. | Ozer, J., et al. 1994. Genes Dev. 8: 2324-35. PMID: 7958899
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TFIIA-γ Antibody (D-6) | sc-374483 | 200 µg/ml | $322.00 | |||
TFIIA-γ Antibody (D-6): m-IgG Fc BP-HRP Bundle | sc-527468 | 200 µg Ab; 10 µg BP | $361.00 | |||
TFIIA-γ Antibody (D-6): m-IgG1 BP-HRP Bundle | sc-532841 | 200 µg Ab; 20 µg BP | $361.00 | |||
TFIIA-γ Antibody (D-6) X | sc-374483 X | 200 µg/0.1 ml | $322.00 | |||
TFIIA-γ Antibody (D-6) AC | sc-374483 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TFIIA-γ Antibody (D-6) HRP | sc-374483 HRP | 200 µg/ml | $322.00 | |||
TFIIA-γ Antibody (D-6) FITC | sc-374483 FITC | 200 µg/ml | $336.00 | |||
TFIIA-γ Antibody (D-6) PE | sc-374483 PE | 200 µg/ml | $349.00 | |||
TFIIA-γ Antibody (D-6) Alexa Fluor® 488 | sc-374483 AF488 | 200 µg/ml | $364.00 | |||
TFIIA-γ Antibody (D-6) Alexa Fluor® 546 | sc-374483 AF546 | 200 µg/ml | $364.00 | |||
TFIIA-γ Antibody (D-6) Alexa Fluor® 594 | sc-374483 AF594 | 200 µg/ml | $364.00 | |||
TFIIA-γ Antibody (D-6) Alexa Fluor® 647 | sc-374483 AF647 | 200 µg/ml | $364.00 | |||
TFIIA-γ Antibody (D-6) Alexa Fluor® 680 | sc-374483 AF680 | 200 µg/ml | $364.00 | |||
TFIIA-γ Antibody (D-6) Alexa Fluor® 790 | sc-374483 AF790 | 200 µg/ml | $364.00 | |||
TFIIA-γ (D-6) Neutralizing Peptide | sc-374483 P | 100 µg/0.5 ml | $69.00 |